PMID: 9168391May 1, 1997Paper

Quantitative characterization of vascular endothelial cell morphology and orientation using Fourier transform analysis

Journal of Biomechanical Engineering
B M Palmer, R Bizios

Abstract

Fourier Transform methods were used to quantify mean elongation, mean orientation, and standard deviation of orientations of cultured vascular endothelial cells. Images of cell populations, which had been subjected to 11 and 20 hours of shear stress at 30 dynes/cm2 and 20 hours of no shear, were analyzed by Fourier Transform methods. Measurements of cell morphology and orientation characteristics were also obtained using a manual method for comparison purposes. The results of the study showed that mean cell orientation can be determined accurately with the Fourier Transform methods. Attempts to determine the standard deviation of cell orientations, however, resulted in poorer estimates of mean elongation and standard deviation of orientations except in the case of exposure of endothelial cells to 20 hours of shear, where the actual standard deviation of orientations was low. When the value for standard deviation of orientations was constrained to zero, a minimum possible mean elongation was determined reliably using the Fourier Transform methods. Use of the Fourier Transform methods in determining morphological and orientation characteristics of cell monolayers is fast and objective and may provide a basis for identifying other...Continue Reading

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Citations

Feb 17, 2006·Annals of Biomedical Engineering·Chee Ping Ng, Melody A Swartz
Jan 26, 2013·Tissue Engineering. Part a·Nathan R SchieleDavid T Corr
Feb 21, 2008·Journal of Biomedical Materials Research. Part a·E A Sander, V H Barocas
Sep 24, 2004·IEEE Transactions on Nanobioscience·Akinori Umeno, Shoogo Ueno
Mar 2, 2002·Journal of Biomechanical Engineering·Ann A LeeWilliam J Karlon
Sep 13, 2017·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Xianghui GongYubo Fan
Mar 10, 2011·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Steven Frank Kemeny, Alisa Morss Clyne
Aug 19, 2009·Optics Express·Raghu Ambekar Ramachandra RaoKimani C Toussaint
May 19, 2017·Tissue Engineering and Regenerative Medicine·Mohsen RabbaniMorteza Teymoori
Mar 18, 2020·Scientific Reports·Kunihiko HashimotoMasaru Ishii
May 29, 2013·Advanced Healthcare Materials·Haiyang YuWinston Shim
Jul 30, 2005·Journal of Biomedical Materials Research. Part a·John D GlaweMichael J McShane

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