Quantitative comparison between full-spectrum and filter-based imaging in hyperspectral fluorescence microscopy.

Journal of Microscopy
Liang GaoTomasz S Tkaczyk

Abstract

We implement a filterless illumination scheme on a hyperspectral fluorescence microscope to achieve full-range spectral imaging. The microscope employs polarisation filtering, spatial filtering and spectral unmixing filtering to replace the role of traditional filters. Quantitative comparisons between full-spectrum and filter-based microscopy are provided in the context of signal dynamic range and accuracy of measured fluorophores' emission spectra. To show potential applications, a five-colour cell immunofluorescence imaging experiment is theoretically simulated. Simulation results indicate that the use of proposed full-spectrum imaging technique may result in three times improvement in signal dynamic range compared to that can be achieved in the filter-based imaging.

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Citations

Jun 7, 2014·Annual Review of Biomedical Engineering·Lihong V Wang, Liang Gao
Aug 31, 2016·International Ophthalmology Clinics·Jason G DwightTomasz S Tkaczyk
Jul 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Hongbin PuDa-Wen Sun

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