PMID: 9178846May 9, 1997Paper

Quantitative comparison of mu opioid receptor mRNA in selected CNS regions of alcohol naive rats selectively bred for high and low alcohol drinking

Neuroscience Letters
J GongL Yu

Abstract

We combined solution hybridization, ribonuclease protection and microdissection techniques to quantitatively compare the anatomical distribution of mu receptor mRNA in discrete brain regions of alcohol naive rats selectively bred for high and low alcohol drinking (HAD and LAD lines, respectively). The solution hybridization assay is highly sensitive and can detect mu opioid receptor mRNA in a 100-fold linear range from 4 to 421 amol. HAD and LAD rats exhibited a similar level of mu receptor mRNA in all central nervous system (CNS) regions examined except for the inferior colliculus. Our data suggest that the steady-state level of mu receptor mRNA is not associated with genetic differences in alcohol drinking behavior.

References

Apr 7, 1977·Nature·J D Belluzzi, L Stein
Jan 1, 1992·Brain Research. Molecular Brain Research·Y S ZhuC E Inturrisi
Jul 1, 1994·The Journal of Comparative Neurology·J M DelfsM F Chesselet
Feb 1, 1993·Clinical Neuropharmacology·G W Pasternak
Jul 1, 1993·Pharmacology, Biochemistry, and Behavior·P Hyytiä
Nov 1, 1996·Alcoholism, Clinical and Experimental Research·J C Froehlich
Mar 25, 1998·Pharmacology, Biochemistry, and Behavior·S Krishnan-SarinJ C Froehlich

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Citations

Dec 3, 1999·Progress in Neuro-psychopharmacology & Biological Psychiatry·M S Cowen, A J Lawrence
Sep 2, 1999·Alcoholism, Clinical and Experimental Research·P FaddaW Fratta
May 26, 2004·Physiology & Behavior·L M Oswald, G S Wand
Oct 1, 2009·Physiological Reviews·Julie Le MerrerBrigitte L Kieffer

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