PMID: 24366627Dec 25, 2013Paper

Quantitative comparison of the autofluorescence of bacteria and polystyrene microspheres under violet wavelength excitation for verification of fluorescence-based bioaerosol detector results

Biocontrol Science
Norio Hasegawa

Abstract

The autofluorescence intensity of bacteria and fungal spores was quantified by fluorescence microscopy in order to obtain the information for evaluating fluorescence-based bioaerosol detectors and was comparable to that of some types of polystyrene microspheres (PSMs). Although the intensity for microbes was distributed across a wide range over an order of magnitude in gray scale, it was in the intensity range of certain PSMs. Furthermore, some of those bacteria and PSMs were aerosolized in a test chamber and the fluorescence intensity was measured with a bioaerosol detector. Although there was a slight difference in the order of intensity from the results obtained by fluorescence microscopy, the fluorescence-based bioaerosol detector showed the intensity was in a comparable range.

References

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