PMID: 8969452Dec 1, 1996

Quantitative conversion of glucose into glucose 6-phosphate by intact Escherichia coli cells

Biotechnology and Applied Biochemistry
J R van der ZeeK J Hellingwerf


The use of intact Escherichia coli cells for the conversion of glucose into glucose 6-phosphate using the Uhp system for transport of the phosphorylated sugar out of the cell was investigated. The strain E. coli DF214, which is not capable of glucose 6-phosphate catabolism via glycolysis or the Entner-Douderoff pathway, was used. The efflux of glucose 6-phosphate was dependent on the presence of UhpT, the hexose-phosphate transporter, plus the presence of the uncoupler carbonyl cyanide m-chlorophenylhydrazone or the ionophore valinomycin. At low glucose concentrations (e.g. 2.5 mM), near-quantitative conversion (> or = 80%) of glucose into extracellular glucose 6-phosphate can be achieved. When cells are incubated for a shorter period of time, complete conversion can occur.

Related Concepts

Alkalescens-Dispar Group
Anhydrous Dextrose
Hydrogen-Ion Concentration
Hexose Transporter

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