PMID: 15392564Nov 1, 1949Paper

Quantitative cytochemical determination of desoxyribonucleic acid with the Feulgen nucleal reaction

The Journal of General Physiology
H RIS, A E MIRSKY

Abstract

The possibility of using the Feulgen nucleal reaction for a quantitative cytochemical estimation of desoxyribonucleic acid (DNA) was investigated. The intensity of the reaction in nuclei was determined by absorption measurements with the microscope. The accuracy of such measurements was tested by comparison with measurements on the same material with a Beckman spectrophotometer. The values obtained with the microscope agreed within a few per cent with those obtained with the Beckman spectrophotometer. Furthermore, the errors introduced by uneven distribution of absorbing material, by variations in the numerical aperture of the system, and by variation in the area used on the phototube were investigated empirically. The following variables were studied with regard to their effect on the intensity of the Feulgen reaction: type of fixation, time of hydrolysis after acetic acid-alcohol and formalin fixation, time of staining in leucobasic fuchsin, method of preparation of leucobasic fuchsin. The intensity of the Feulgen reaction in liver and erythrocyte nuclei of various vertebrates, fixed in acetic acid-alcohol, was then compared with the DNA content of these nuclei as determined by chemical analysis on a known number of nuclei. T...Continue Reading

References

Mar 1, 1948·Proceedings of the National Academy of Sciences of the United States of America·H S Di Stefano
Mar 20, 1949·The Journal of General Physiology·H RIS, A E MIRSKY
Apr 2, 1949·Nature·C F LIW G OVEREND
Apr 30, 1949·Nature·A E MIRSKY, H RIS

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