Quantitative cytological studies of spermatogenesis in intact and hypophysectomized rats: identification of androgen-dependent stages

Endocrinology
Y T SunD M de Kretser

Abstract

A stereological study of the numbers of germ cells in various stages of spermatogenesis was undertaken in testosterone-treated intact and hypophysectomized (HPX) rats. Adult Sprague-Dawley rats were given testosterone by Silastic implants, which either inhibited (3-cm length) or partially maintained (10 cm) spermatogenesis over a 13-week period. The numbers of nuclei of the various germ cell categories (spermatogonia, spermatocytes, and round spermatids) in the testes were estimated by profile counting and measurement of nuclear diameter. The numbers of elongated spermatids were determined separately in testicular homogenates. Testis weight, seminiferous tubule volume, and tubule diameter were significantly decreased in intact rats with 3- and 10-cm testosterone implants and in HPX rats, although they were partially maintained in groups with 10-cm implants compared to those in groups with 3-cm implants (P less than 0.05). The effect of 3-cm testosterone implants in the intact group was to suppress the number of spermatogonia to 57%, reduce the conversion of spermatogonia to spermatocytes to 85%, and reduce the conversion of round to elongated spermatids to 19% of the control value. This latter effect was largely overcome with 1...Continue Reading

Citations

May 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S M Harrison, S Roffler-Tarlov
Jan 1, 1995·Developmental Genetics·M A HandelT Wiltshire
Apr 1, 1995·Trends in Endocrinology and Metabolism : TEM·R I McLachlanD M de Kretser
Sep 16, 2000·Molecular and Cellular Endocrinology·J TesarikE Greco
Apr 1, 1992·Baillière's Clinical Endocrinology and Metabolism·D M de KretserN G Wreford
Feb 10, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Jul 2, 2003·Reproductive Biology and Endocrinology : RB&E·D Antony JeyarajPeter Petrusz
Jul 1, 2011·Drug and Chemical Toxicology·Pinar ErkekogluFiliz Hincal
Jul 29, 2009·Behavioural Processes·Małgorzata Kruczek, Józefa Styrna
Sep 1, 2007·Environmental Toxicology and Pharmacology·Amar K ChandraMahitosh Sarkar
Nov 26, 2005·Environmental Research·Britta SelzsamIbrahim Chahoud
Mar 7, 2014·Seminars in Cell & Developmental Biology·Peter J O'Shaughnessy
Mar 8, 2006·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Marilise M HornThemis R Silveira
Jul 2, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Nour NicolasMark P Hedger
Feb 18, 1998·Archives of Andrology·R GunasegaramS S Ratnam
Mar 29, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Monika FijakAndreas Meinhardt
May 1, 2014·Spermatogenesis·Suresh Ramaswamy, Gerhard F Weinbauer
May 1, 2021·Biology of Reproduction·Millissia Ben MaamarMichael K Skinner

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