Quantitative description of analyte migration behavior based on dynamic complexation in capillary electrophoresis with one or more additives

Electrophoresis
X PengD D Chen

Abstract

A comprehensive theory is proposed to describe the migration behavior of analytes in capillary electrophoresis (CE) when one or more additives are present in the buffer solution. This theory amalgamates and extends the previous work done by others. The capacity factor (k') in this theory is defined as the product of the equilibrium constant and the additive concentration, thus, k' changes linearly with additive concentration. The net electrophoretic mobility of an analyte is a function of k', therefore, it can be changed by varying the additive concentration. Three parameters are needed to predict the mobility of an analyte in a one-additive CE system: the mobility of the free analyte, the mobility of the complex, and the equilibrium constant for the analyte-additive interaction (which determines the fraction of the free analyte at different additive concentrations). When additives are used, the change in viscosity obscures this relationship, therefore, a viscosity correction factor is required to convert all mobilities to an ideal state where the viscosity remains constant. The migration behavior of an analyte in a solution with multiple additives can be predicted and controlled, once the equilibrium constants of the interacti...Continue Reading

References

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Citations

Apr 29, 1998·Electrophoresis·M T Bowser, D D Chen
Apr 10, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Niels H H Heegaard, Robert T Kennedy
Feb 5, 2003·Current Opinion in Biotechnology·Chiara Galbusera, David D Y Chen
Mar 21, 2008·Analytical Chemistry·Bryan R Fonslow, Michael T Bowser
Jan 28, 2014·Journal of Chromatography. a·Ludmila MüllerováBohuslav Gaš
Sep 13, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jirasak ThreepromJin-Ming Lin
Apr 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jesper Østergaard
Jun 12, 2009·Electrophoresis·Niels H H Heegaard
Nov 8, 2005·Electrophoresis·Amy M MacDonaldCharles A Lucy
Sep 28, 2007·Electrophoresis·Simo P Porras, Matti Jussila
Aug 26, 2016·Analytical and Bioanalytical Chemistry·Pavel DubskýMartin Ansorge
Mar 29, 2002·Electrophoresis·Niels H H HeegaardDavid D Y Chen
Mar 29, 2002·Electrophoresis·Philip Britz-McKibbin, David D Y Chen

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