Quantitative detection of Borrelia burgdorferi in 2-millimeter skin samples of erythema migrans lesions: correlation of results with clinical and laboratory findings.

Journal of Clinical Microbiology
Dionysios LiverisIra Schwartz

Abstract

Variability of disease manifestations has been noted in patients with Lyme disease. A contributing factor to this variation may be the number of spirochetes present in infected patients. We evaluated clinical and laboratory findings for patients with erythema migrans with regard to the number of Borrelia burgdorferi organisms detected by quantitative PCR (qPCR) in 2-mm skin biopsy specimens. B. burgdorferi was detected in 80% (40 of 50) of the specimens tested; the mean number of spirochetes in these specimens ranged over 3 orders of magnitude (10 to 11,000 spirochetes per 2-mm biopsy specimen). Larger numbers of spirochetes were significantly associated with a shorter duration of the erythema migrans skin lesion (P = 0.020), smaller skin lesions (P = 0.020), and infection with a specific genotype of B. burgdorferi (P = 0.008) but not with the number or severity of symptoms. Skin culture positivity was significantly associated with skin lesions containing larger numbers of spirochetes (P = 0.019).

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Citations

Aug 16, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D LiverisG P Wormser
Apr 29, 2010·Current Allergy and Asthma Reports·Adriana R Marques
Nov 16, 2012·The New England Journal of Medicine·Robert B NadelmanGary P Wormser
Oct 15, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Raphael B StrickerJoseph J Burrascano
Jan 26, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Gary P WormserIra Schwartz
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Jul 9, 2008·Infection and Immunity·Dionysios LiverisIra Schwartz
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Apr 3, 2004·Journal of Bacteriology·Dionysios LiverisIra Schwartz
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