Quantitative detection of Toxoplasma gondii in tissues of experimentally infected turkeys and in retail turkey products by magnetic-capture PCR

Food Microbiology
Martin KoetheMartina Ludewig

Abstract

Magnetic-capture PCR was applied for the quantitative detection of Toxoplasma gondii in tissues of experimentally infected turkeys and retail turkey meat products. For experimental infection, three T. gondii strains (ME49, CZ-Tiger, NED), varying infectious doses in different matrices (organisms in single mouse brains or 10(3), 10(5), or 10(6) oocysts in buffer) were used. From all animals, breast, thigh, and drumstick muscle tissues and for CZ-Tiger-infected animals additionally brains and hearts were analyzed. Using the magnetic-capture PCR large volumes of up to 100 g were examined. Our results show that most T. gondii parasites are present in brain and heart tissue. Of the three skeletal muscle types, drumsticks were affected at the highest and breast at the lowest level. Type III strain (NED) seems to be less efficient in infecting turkeys compared to type II strains, because only few tissues of NED infected animals contained T. gondii DNA. Furthermore, the number of detected parasitic stages increased with the level of infectious dose. Infection mode by either oocyst or tissue cyst stage did not have an effect on the amount of T. gondii present in tissues. In retail turkey meat products T. gondii DNA was not detectable al...Continue Reading

References

Oct 1, 1985·Journal of Wildlife Diseases·E W Howerth, N Rodenroth
Dec 1, 1995·The Journal of Infectious Diseases·D K Howe, L D Sibley
Nov 1, 1993·Antimicrobial Agents and Chemotherapy·A P Ricketts, E R Pfefferkorn
Apr 1, 1995·Journal of Wildlife Diseases·C F QuistW R Davidson
Oct 8, 1999·Veterinary Parasitology·I Esteban-RedondoE A Innes
Jun 23, 2000·The Journal of Parasitology·A El-MassryJ P Dubey
Jun 1, 1993·Avian Pathology : Journal of the W.V.P.A·I Literak, K Hejlicek
Dec 1, 2000·Avian Pathology : Journal of the W.V.P.A·K Sedlak, I L Franti
Feb 17, 2009·International Journal for Parasitology·Louis M Weiss, Jitender P Dubey
Nov 26, 2009·Avian Pathology : Journal of the W.V.P.A·Eva BártováIvan Literák
Mar 31, 2010·International Journal of Food Microbiology·Marieke OpsteeghJoke van der Giessen
Apr 24, 2013·Veterinary Parasitology·B BangouraA Daugschies

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Citations

Oct 7, 2017·Parasitology Research·Lysanne HiobB Bangoura
Feb 26, 2020·Food and Waterborne Parasitology·S StelzerG Schares
Nov 6, 2020·Research in Veterinary Science·S Almeria, J P Dubey

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