Quantitative determination of acetylcholinesterase by enzyme antigen immunoassay: methodological aspects and clinical use

Scandinavian Journal of Clinical and Laboratory Investigation
J HangaardB Nørgaard-Pedersen

Abstract

We here describe the optimization of an immunochemical measuring method for acetylcholinesterase (AChE) found in different human body fluids. The principle is the binding of a polyclonal antibody to a solid support (microtitre plate), followed by quantitation of the enzymatic active antigen, by its own enzymatic activity. The test is mainly thought as a diagnostic tool for the prenatal detection of neural tube defects (NTD). The assay is optimized with respect to antibodies and a number of physical parameters. The test is accurate and reliable, and it yields quantitative results. Further we show it to give neither false positive nor false negative values in the prediction of NTD. The assay also performs well on other sample-materials. We suggest that the test will be routinely used alongside amniotic alpha-fetoprotein (AFP) determinations to ensure the correct prenatal diagnosis of NTD.

References

Jun 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·G DaleJ Wagget
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N Harboe, A Ingild
Jan 1, 1967·Humangenetik·K AltlandH W Goedde
Mar 28, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·P K BuamahA M Ward
Dec 1, 1980·British Journal of Obstetrics and Gynaecology·M J Seller, K J Cole
Nov 20, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·D J Brock, C Hayward
Feb 1, 1982·British Journal of Obstetrics and Gynaecology·A P ReadR Harris
Oct 1, 1982·British Journal of Obstetrics and Gynaecology·R D BarlowC H Rodeck
Jun 1, 1980·British Journal of Obstetrics and Gynaecology·M J SellerP E Polani
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

❮ Previous
Next ❯

Citations

Jul 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·K SorensenB Norgaard-Pedersen
Jun 30, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·A G RasmussenB Norgaard-Pedersen
Jan 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·K SorensenB Norgaard-Pedersen
Mar 23, 1985·Lancet·B Nørgaard PedersenU Brodbeck
Oct 1, 1989·Scandinavian Journal of Clinical and Laboratory Investigation·A G RasmussenS O Larsen
Jun 1, 1991·Scandinavian Journal of Clinical and Laboratory Investigation·J HangaardB Nørgaard-Pedersen
Jan 1, 1986·Acta paediatrica Scandinavica·C G Bergstrand
Mar 18, 1987·Biochimica Et Biophysica Acta·K SorensenB Norgaard-Pedersen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.