Quantitative determination of clonazepam in plasma by gas chromatography-negative ion chemical ionization mass spectrometry

Journal of Chromatography. B, Biomedical Applications
D SongK Kohlhof

Abstract

A gas chromatographic-negative ion chemical ionization mass spectrometric (GC-NCI-MS) method for the quantitative analysis of clonazepam in human plasma is described. Clonazepam (M(r) = 315) was derivatized by N,O-bis-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane. A pre-conditioning procedure involving injection of a silyl-8 and ethyl acetate extraction solution from plasma reduces the interaction between clonazepam-TMS and the analytical system. The routine limit of quantification was set to be 0.25 ng/ml with an injection volume of 2 microliters and a sample volume of 1 ml. The signal-to-noise ratio was greater than five. The detection limit for clonazepam can reach 0.1 ng/ml. The isotope clonazepam-d5 was used as the internal standard.

Citations

Jan 29, 2013·Journal of Chromatographic Science·Mohammad Nasir UddinIoannis N Papadoyannis
Dec 4, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Hugo M NeelsWilly E Lambert
Jul 1, 2006·Forensic Science International : Synergy·Jeffery Hackett, Albert A Elian
Jan 23, 2014·Journal of Separation Science·Homayon Ahmad PanahiElham Moniri
Jan 30, 2002·Journal of Pharmaceutical and Biomedical Analysis·M M Hefnawy
Aug 10, 2000·Journal of Pharmaceutical and Biomedical Analysis·A El Mahjoub, C Staub

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