Quantitative determination of corticosteroids in bovine milk using mixed-mode polymeric strong cation exchange solid-phase extraction and liquid chromatography-tandem mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis
Adám TölgyesiJeno Fekete

Abstract

A new method was developed to identify and quantify corticosteroids (prednisolone, methylprednisone, flumetasone, dexamethasone, and methylprednisolone) in raw bovine milk by liquid chromatography-tandem mass spectrometry (LC-MS/MS) utilizing mixed-mode polymeric strong cation exchange and reversed-phase (MCX) solid-phase extraction (SPE) to reduce ion effects in a multimode ion (MMI) source. The main advantage of this method over other commonly used methods includes the use of a single SPE cartridge with a low volume for sample preparation and fast separation on the HPLC system with reduced ion suppression. This study is the first to report the determination of methylprednisone, a metabolite of methylprednisolone, in bovine milk. This method was validated in accordance with the European Union (EU) Commission Decision 2002/657/EC. The recoveries vary between 90% and 105%. The within-laboratory reproducibility (precision) is less than 30%. The decision limits and detection capabilities were calculated along with LODs, which ranged from 0.02 to 0.07 microg/kg. The method was further enhanced by its successful adaptation to other LC-MS/MS systems equipped with the newly developed ion source, Agilent Jet Stream (AJS). After optimiz...Continue Reading

References

May 2, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Man Ho ChoiBong Chul Chung
May 7, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Giuliano NeriGastone G Nussdorfer
Apr 29, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marc CherletPatrick De Backer
Nov 29, 2008·Journal of Pharmaceutical and Biomedical Analysis·Szabolcs FeketeKatalin Ganzler
Jan 17, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yi YangHejun Duan
Mar 11, 2009·Journal of Chromatography. a·H F De BrabanderS Croubels
Mar 31, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Didier OrtelliPatrick Edder
Jul 15, 2009·Journal of Chromatography. a·Petra Gowik
Sep 29, 2009·Journal of Chromatography. a·Brian KinsellaMartin Danaher
May 12, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Adám TölgyesiJeno Fekete

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Citations

May 29, 2016·Journal of Pharmaceutical and Biomedical Analysis·Kelly Zhang, Xiaodong Liu
Sep 21, 2016·Journal of Pharmaceutical and Biomedical Analysis·Kelly Zhang, Xiaodong Liu
Apr 12, 2017·Critical Reviews in Analytical Chemistry·Justyna Płotka-WasylkaJacek Namieśnik
May 12, 2011·Analytical and Bioanalytical Chemistry·Ilse BecueCarlos Van Peteghem
Dec 19, 2018·Journal of Analytical Toxicology·Fernando X MoreiraPaula G de Pinho

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