Quantitative determination of free amino acids in the hemolymph of autogenous and anautogenous strains of Culex tarsalis (Diptera: Culicidae)

Journal of Medical Entomology
T Su, M S Mulla

Abstract

Autogenous and anautogenous strains were selected from the egg rafts of Culex tarsalis Coquillett collected from Coachella Valley, California. In autogenous (21-25 generations) and anautogenous (0-4 generations) strains used for hemolymph free amino acid analysis, the autogenous oviposition rates were 73.6-83.6% and 2.7-3.9%, the proportions of females undergoing autogenous ovarian development 120 h postemergence were 75.6 and 8.9%, respectively. To study the presence and quantities of free amino acids and explore their relationship to autogenous ovarian development, the 6 legs of females from both autogenous and anautogenous strains were removed at the coxo-femoral joints, and hemolymph was collected by centrifuging the bodies of 50-100 females. Proteins in the hemolymph were precipitated with 10% sulphosalicylic acid. On days 1, 4, 7, and 10 postemergence, a total of 17 free amino acids was found in females from the autogenous strain, and a total of 16 was found in females from the anautogenous strain in which asparagine (Asn) was absent. Comparisons of free amino acid concentrations between these 2 strains indicated that there were significant differences in total free amino acids and 13 individual free amino acids except Gl...Continue Reading

Citations

Feb 10, 2004·Journal of Medical Entomology·L E HugoP A Ryan
May 17, 2005·Insect Biochemistry and Molecular Biology·Geoffrey M AttardoAlexander S Raikhel
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Geoffrey M AttardoAlexander S Raikhel
Jan 6, 2016·Archives of Insect Biochemistry and Physiology·Magdalena Bil, Roger Huybrechts
Aug 5, 2006·The Journal of Experimental Biology·Geoffrey M AttardoAlexander S Raikhel

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