PMID: 9185169Jul 1, 1997Paper

Quantitative determination of glutamate turnover by 1H-observed, 13C-edited nuclear magnetic resonance spectroscopy in the cerebral cortex ex vivo: interrelationships with oxygen consumption

Neurochemistry International
J LukkarinenR A Kauppinen

Abstract

The kinetics of glutamate 13C-4 label appearance from D-[1-13C]-glucose and 13C-4 label disappearance from steady state following D-12C-glucose incubation were quantified with 1H-observed, 13C-edited nuclear magnetic resonance (NMR) spectroscopy in the superfused brain slices under largely varying oxygen consumption. Label incorporation to and from glutamate C-4 were fitted into mono- or bicompartmental models in order to determine the respective rate constants and to assess the presence of plausible multiple pools. At a steady-state oxygen consumption of approximately 4 mumol/min/g dry weight, glutamate labelling could be fitted into a biexponential equation, suggesting that there were two compartments with a large difference in their rates (respective rate constants of 0.022 and 0.149) and pool sizes (relative contributions of 91.2 and 8.8%, respectively). Stimulation of oxygen consumption in the brain slice preparations with either 40 mM KCl by 59.5 +/- 10.3% or 5 microM carbonyl cyanide m-fluorophenyl hydrazone by 61.4 +/- 8.4% increased glutamate C-4 labelling rate constants to 0.058 +/- 0.009 and 0.054 +/- 0.006, respectively. In the stimulated slice preparation, glutamate labelling could only be fitted into a monoexponen...Continue Reading

References

May 1, 1992·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·G F MasonR G Shulman
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D L RothmanR G Shulman
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Feb 15, 1994·The Biochemical Journal·R A KauppinenS R Williams

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Citations

Sep 16, 2010·Neurochemical Research·Ursula Sonnewald, Caroline Rae
May 22, 2002·Neurochemistry International·Rolf Gruetter
Feb 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Myriam M ChaumeilVincent Lebon
Mar 4, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J M HakumäkiR A Kauppinen
Aug 18, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Miguel Martínez-MaestroHarald E Möller
Jun 17, 1999·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J PfeufferR Gruetter

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