Quantitative determination of myricetin in rat plasma by ultra performance liquid chromatography tandem mass spectrometry and its absolute bioavailability

Drug Research
Y DangG Ji

Abstract

Myricetin is a widely distributed bioactive flavonoid with scientific interest attributed to its anti-oxidant, antitumor, and anti-inflammatory properties. A specific and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed and validated for identification and quantification of myricetin in rat plasma after oral and intravenous administrations. Kaempferol was used as an internal standard. Followed by β-glucuronidase and sulfatase hydrolysis and liquid-liquid extraction with ethyl acetate, the analytes were separated on an Acquity UPLC BEH C18 column (2.1×50 mm, 1.7 μm) and analyzed in the selected ion recording with a negative electrospray ionization mode. The developed method was validated for selectivity, accuracy, precision, linearity, recovery, stability and matrix effect. The assay was validated over a wide concentration range of 2-4,000 ng/mL. Intra- and inter-day precisions were all less than 13.49% and accuracy ranged from 95.75 to 109.80%. The present method was successfully applied to investigate a pharmacokinetic study of myricetin following intravenous and oral administrations to rats. The absolute bioavailability was found to be 9.62% and 9.74% at 2 oral ...Continue Reading

Citations

Mar 26, 2016·The Journal of Nutritional Biochemistry·Mingdao MuFudi Wang
May 20, 2015·AAPS PharmSciTech·Stephen J Franklin, Paul B Myrdal
Feb 27, 2016·Basic & Clinical Pharmacology & Toxicology·Enayatollah SeydiJalal Pourahmad
Mar 3, 2020·Frontiers in Pharmacology·Renato Simões GasparAntonio Marcus de Andrade Paes

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