Quantitative determination of perfluorooctanoic acid ammonium salt in human serum by high-performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Cristina Sottani, Claudio Minoia

Abstract

A sensitive, specific, accurate and reproducible analytical method was developed and validated to quantify perfluorooctanoic acid (PFOA) in human serum. After initial extraction with an ion-paring reagent, the procedure for quantifying PFOA is based on high-performance liquid chromatography (HPLC) interfaced to negative ion tandem mass spectrometry, operating in selected ion monitoring mode. The retention times of PFOA and its internal standard (D,L-malic acid) were 5.85 and 1.70 min, respectively. The assay was linear over the range 0-500 ng/mL, with a lower limit of quantification (LOQ) of 25 ng/mL, and with a coefficient of variation (CV) of 7.3%. The lower limit of detection (LOD) was assessed as 10 ng/mL. The overall precision and accuracy were assessed on three different days. The within- and between-day precision was < or =9.7 and 6.8%, respectively, and the accuracy was in the range 96-114%. The mean extracted recovery assessed at three different concentrations (100, 250, and 500 ng/mL) was always more than 85%. With this method no derivatization procedure was needed, thus avoiding possible thermal and chemical decomposition reactions of PFOA. The assay was applied to quantify perfluorooctanoic acid in serum from employ...Continue Reading

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Citations

May 20, 2006·Analytical and Bioanalytical Chemistry·Marta VillagrasaDamià Barceló
Feb 5, 2015·Mass Spectrometry Reviews·Zachary S BreitbachDaniel W Armstrong
Mar 15, 2005·Electrophoresis·Lena WójcikMarek Trojanowicz
Jul 9, 2004·Toxicology and Applied Pharmacology·Christopher LauJohn M Rogers
Sep 27, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·W Matthew HendersonMary Alice Smith
Apr 19, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·John M FlahertyBogdan Szostek
Jul 12, 2002·Journal of Mass Spectrometry : JMS
Dec 28, 2019·International Journal of Environmental Research and Public Health·Zhen Li, Hongwei Sun
Jun 15, 2004·Analytical Chemistry·Susan D Richardson
Jan 27, 2005·Environmental Science & Technology·Amila O De Silva, Scott A Mabury
Aug 7, 2004·Environmental Science & Technology·Zsuzsanna KuklenyikAntonia M Calafat
Jul 13, 2006·The Journal of Physical Chemistry. a·Paul J KrusicRobert C Buck

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