Quantitative determination of platelet surface alloantigens using a monoclonal probe.

Human Immunology
M JansonR H Aster

Abstract

A monoclonal antibody with specificity for the Fc portion of IgG was used to determine the number of IgG alloantibody molecules bound at saturation to alloantigens of the PlA1 and HLA systems on normal human platelets. In preliminary studies, it was found that the number of cell-bound IgG molecules recognized by this probe correlates well with the number measured by electroimmunoassay, an independent measure of alloantibody binding. PlA1-positive platelets could be divided into two groups binding 34,000-43,000 or 19,000-24,000 alloantibody molecules. Family studies and studies with a cytolytic assay showed that the former group is homozygous and the latter heterozygous for PlA1. Because the number of glycoprotein IIIa (GPIIIa) molecules carrying the PlA1 determinant on the surface of normal platelets is thought to be about 40,000, these findings suggest that each GPIIIa molecule carries one PlA1 determinant. The number of class I HLA molecules expressed on normal platelets was considerably smaller than the number of PlA1 determinants, ranging from 4400 to 10,000 (HLA-A2), 870 to 8400 (Bw4), and 1300 to 5800 (Bw6). Preliminary analysis indicates that stronger or weaker expression of Bw4 and of Bw6 correlates with certain "privat...Continue Reading

References

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Citations

Oct 1, 1990·Transfusion Medicine Reviews·A E von dem BorneR W Kuijpers
Apr 1, 1987·Transfusion Medicine Reviews·T J KunickiD J Nugent
Feb 7, 2013·British Journal of Haematology·Julie A PetersonRichard H Aster
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Oct 13, 2000·Baillière's Best Practice & Research. Clinical Haematology·V S BlanchetteM Rand
Dec 1, 1991·American Journal of Hematology·J Freedman, A Hornstein
Jun 18, 2019·Journal of Thrombosis and Haemostasis : JTH·Catherine AngénieuxBlandine Maître
Oct 14, 2020·Transfusion·Rachel K HortonJustin E Juskewitch
Jul 1, 1989·Baillière's Clinical Haematology·A E von dem Borne, W H Ouwehand

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