PMID: 3769524Oct 1, 1986Paper

Quantitative determination of S-antigen in human ocular tissues, aqueous humour and serum

Current Eye Research
J ZaalA Kijlstra

Abstract

Retinal S-antigen is thought to play an important role in the immunopathogenesis of uveitis. To investigate whether S-antigen is a sequestered antigen confined to the retina, a sensitive ELISA was developed to determine the levels of this protein in various human ocular tissues, aqueous humour and serum. The ELISA was performed by incubating S-antigen-containing samples with solid-phase bound immunospecific rabbit anti human S-antigen F(ab')2 fragments and then incubating the bound S-antigen with mouse anti bovine S-antigen serum and the bound mouse antibodies with peroxidase-labelled rabbit anti mouse IgG; the peroxidase activity is developed with ABTS. This method was demonstrated to be highly sensitive and specific: S-antigen could be measured at concentrations of 5-10 nanograms per ml, irrespective of whether it was present in buffer, undiluted whole serum of tissue extracts. Human retinas were shown to contain approximately 1.2 mg immunoreactive S-antigen per retina. Of the other human ocular tissues, only the vitreous and choroid (including pigment epithelium) contained small amounts of S-antigen. Low levels of S-antigen could also be detected in the aqueous humour of two out of seven patients with posterior uveitis. No i...Continue Reading

References

May 1, 1985·Current Eye Research·R M BroekhuyseH J Winkens
Jan 1, 1985·Current Eye Research·R M BroekhuyseA J Verkley
May 1, 1985·Experimental Eye Research·R M Broekhuyse, H J Bessems
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, A Kawaoi
Jun 1, 1984·Current Eye Research·J P FaureC Boucheix
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