Quantitative determination of six major triterpenoids in Ganoderma lucidum and related species by high performance liquid chromatography

Journal of Pharmaceutical and Biomedical Analysis
Xiaoming WangDe-An Guo

Abstract

A reversed-phase liquid chromatographic method was developed for the quantitative determination of six triterpenoids, namely ganoderic acids C2, B, AM1, K, H and D in Ganoderma lucidum and its related species. Samples were extracted with chloroform in ultrasonic bath. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C18 column (250 mmx4.6 mm, 5 microm), with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.0 ml/min, detected at 252 nm. All calibration curves showed good linearity (r2>0.999) within test ranges. The relative deviation of this method was less than 2% for intra- and inter-day assays, and the percentage recovery of the method was 93-103%, with relative standard deviation (R.S.D.) less than 5%. The current assay method was applied to quantitative determination of constituents of triterpenoids in 36 different samples of G. lucidum and its related species. The results indicated that the developed method could be readily utilized as a quality control method for G. lucidum and related species.

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Citations

Mar 24, 2007·Journal of the American Society for Mass Spectrometry·Min YangDe-An Guo
Feb 2, 2012·Chemistry Central Journal·Guang-Ping LvShao-Ping Li
Mar 19, 2011·Science China. Life Sciences·Lishun Wang, Guoqiang Chen
Jan 23, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Q-X YueD-A Guo
Oct 23, 2008·Biomedical Chromatography : BMC·Chun-Mei FuKelvin Sze-Yin Leung
Jan 17, 2012·Proteomics·Li-Shun WangGuo-Qiang Chen
Dec 7, 2011·Journal of Basic Microbiology·R L Toh ChoonM N Siti Mariam
Jan 26, 2012·Phytotherapy Research : PTR·Hui LuLiang Feng
Aug 15, 2006·Phytochemistry·R Russell M Paterson
Aug 25, 2015·Chinese Medicine·Baosheng LiaoShilin Chen
Aug 6, 2013·Carbohydrate Polymers·Ding-Tao WuShao-Ping Li
May 16, 2015·Phytochemistry·Sabulal BabyBalaji Govindan
Nov 23, 2006·Journal of Pharmaceutical and Biomedical Analysis·Xiao-Ming WangDe-An Guo
Jan 15, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Do Thi HaNguyen Phuong Dai Nguyen
Aug 13, 2008·Journal of Asian Natural Products Research·Shu-Hong GuanDe-An Guo
Jul 12, 2018·Journal of Analytical Methods in Chemistry·Hui ZhangJizhong Yan
Sep 6, 2018·Applied Microbiology and Biotechnology·Kai-Di Hsu, Kuan-Chen Cheng
Sep 1, 2015·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Nebojša V KladarBiljana N Božin
Apr 1, 2009·Journal of Natural Products·Naoto SatoMasao Hattori
Apr 18, 2019·Journal of Agricultural and Food Chemistry·Yan-Li WuLi-Xia Chen

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