Quantitative determination of the antitumor alkyl ether phospholipid edelfosine by reversed-phase liquid chromatography-electrospray mass spectrometry: application to cell uptake studies and characterization of drug delivery systems

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
M J Blanco-PríetoF Mollinedo

Abstract

Edelfosine is a synthetic alkyl ether phospholipid that represents a promising class of antitumor agents. However, analytical methods to measure these type compounds are scarce. The lack of a reliable methodology to quantify edelfosine is a major problem in ongoing and scheduled preclinical and clinical trials with this drug. We evaluated the applicability of high-performance liquid chromatography-mass spectrometry to determine edelfosine in biological samples and polymeric delivery systems. Sample pre-treatment involved polymer precipitation or cell lysis with methanol. HPLC separation was performed on an Alltima RPC(18) narrow-bore column and edelfosine quantification was done by electrospray ionization mass spectrometry (ESI-MS) using positive ion mode and selected ion monitoring. Assays were linear in the tested range of 0.3-10 microg/ml. The limit of quantification was 0.3 ng/sample in both matrices, namely biological samples and polymeric delivery systems. The interassay precision ranging from 0.79 to 1.49%, with relative errors of -6.7 and 12.8%. Mean extraction recovery was 95.6%. HPLC-ESI-MS is a reliable system for edelfosine analysis and quantification in samples from different sources, combining advantages of full a...Continue Reading

Citations

Aug 21, 2007·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ander Estella-Hermoso de MendozaMaría J Blanco-Prieto
Apr 27, 2010·Oncogene·F MollinedoM J Blanco-Prieto
Feb 4, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ander Estella-Hermoso de MendozaMaría J Blanco-Prieto
Mar 18, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Faustino MollinedoMaria J Blanco-Prieto

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