Quantitative determination of vitamin D metabolites in plasma using UHPLC-MS/MS.

Analytical and Bioanalytical Chemistry
Shujing DingDietrich A Volmer

Abstract

Vitamin D is an important determinant of bone health at all ages. The plasma concentrations of 25-hydroxy vitamin D (25-OH D) and other metabolites are used as biomarkers for vitamin sufficiency and function. To allow for the simultaneous determination of five vitamin D metabolites, 25-OH D(3), 25-OH D(2), 24,25-(OH)(2) D(3), 1,25-(OH)(2) D(3), and 1,25-(OH)(2) D(2), in low volumes of human plasma, an assay using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was established. Plasma samples were spiked with isotope-labeled internal standards and pretreated using protein precipitation, solid-phase extraction (SPE) and a Diels-Alder derivatization step with 4-phenyl-1,2,4-triazoline-3,5-dione. The SPE recovery rates ranged from 55% to 85%, depending on the vitamin D metabolite; the total sample run time was <5 min. Mass spectrometry was conducted using positive ion electrospray ionization in the multiple reaction monitoring mode on a quadrupole-quadrupole-linear ion trap instrument after pre-column addition of methylamine to increase the ionization efficiency. The intra- and inter-day relative standard deviations were 1.6-4.1% and 3.7-6.8%, respectively. The limit of quantitation for these com...Continue Reading

References

Mar 7, 2000·Journal of Pharmaceutical and Biomedical Analysis·M D Luque de CastroJ M Quesada
Feb 17, 2001·Steroids·J P van LeeuwenH A Pols
Jan 5, 2002·Journal of Chromatography. a·K ShimadaT Higashi
Jul 9, 2004·The Journal of Clinical Endocrinology and Metabolism·N BinkleyM K Drezner
May 18, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Songmei GaoH T Karnes
Jun 14, 2005·American Journal of Physiology. Renal Physiology·Adriana S DussoEduardo Slatopolsky
Apr 1, 2006·Clinical Chemistry·Gary L LensmeyerMarc K Drezner
Jan 2, 2007·The Journal of Steroid Biochemistry and Molecular Biology·G D CarterJ L Berry
Mar 6, 2008·The Journal of Clinical Endocrinology and Metabolism·N BinkleyM K Drezner
Aug 12, 2008·The American Journal of Clinical Nutrition·Ann PrenticeInez Schoenmakers
Aug 12, 2008·The American Journal of Clinical Nutrition·Karen W Phinney
Dec 24, 2008·Annals of Clinical Biochemistry·William D Fraser
Dec 24, 2008·Annals of Clinical Biochemistry·Eric S Kilpatrick
Feb 24, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Darryl EylesJohn McGrath
Apr 4, 2009·Clinical Chemistry·Catherine Massart, Jean-Claude Souberbielle
Sep 2, 2009·Annals of Clinical Biochemistry·Derek McKillopPeter Sharpe
Oct 24, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Carsten S HøjskovHolger J Møller
Jan 16, 2010·Methods in Molecular Biology·Ravinder J Singh
Apr 13, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Johannes M W van den OuwelandHenny van Daal

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Citations

Jun 19, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jennifer F Lai, Adrian A Franke
Jul 3, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tristan E LipkieMario G Ferruzzi
Sep 8, 2011·The British Journal of Nutrition·Kerry S JonesAnn Prentice
Sep 9, 2011·Bioanalysis·Mihaela L Musteata, Florin Marcel Musteata
Dec 24, 2011·Bioanalysis·Pan DengDafang Zhong
Apr 16, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Laura BergadàXavier Matias-Guiu
Mar 30, 2013·Reviews in Endocrine & Metabolic Disorders·Johannes M W van den OuwelandSilvia Bächer
Dec 11, 2013·Bioanalysis·Fabio P GomesAmitha K Hewavitharana
Jul 9, 2014·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Tom J HazellHope A Weiler
Dec 18, 2013·Bioanalysis·Mariëtte Theodora Ackermans, Erik Endert
Nov 26, 2013·Chromatographia·Judyta Cielecka-PiontekPiotr Garbacki
Feb 13, 2016·Scientific Reports·Miriam J MüllerDietrich A Volmer
Feb 20, 2016·Bioanalysis·Nur Sofiah Abu KassimAmitha K Hewavitharana
Jan 15, 2013·Journal of Pharmaceutical and Biomedical Analysis·Xiaomei ZhuangHua Li
Aug 19, 2014·Journal of Clinical Laboratory Analysis·Jos P M WieldersChristian Vogl
Dec 29, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fabio P GomesAmitha K Hewavitharana
Jun 21, 2015·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Gregory WallaceStella M Davies
Sep 22, 2012·Mass Spectrometry Reviews·Rose M GathunguPaul Vouros
Feb 14, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Caroline S StokesFrank Lammert
Jan 21, 2015·Journal of Mass Spectrometry : JMS·Timon GeibDietrich A Volmer
Apr 23, 2014·Scandinavian Journal of Clinical and Laboratory Investigation·Anders BurildJette Jakobsen
Oct 11, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Tatsuya Higashi, Shoujiro Ogawa
Jan 19, 2012·Journal of Obesity·Christian L RothAndrew N Hoofnagle
Oct 26, 2016·Analytical Chemistry·Kevin D ClarkJared L Anderson
Jul 1, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Markus HerrmannEtienne Cavalier
Jun 3, 2014·The Journal of Clinical Endocrinology and Metabolism·K S JonesI Schoenmakers

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