Quantitative enantioseparation of amino acids by comprehensive two-dimensional gas chromatography applied to non-terrestrial samples

Journal of Chromatography. a
Iuliia MyrgorodskaUwe J Meierhenrich

Abstract

This work presents an improved analytical procedure for the resolution and quantification of amino acid enantiomers by multidimensional gas chromatography. The procedure contains a derivatization step, by which amino acids were transformed into N(O,S)-ethoxycarbonylheptafluorobutyl esters. It was optimized for the resolution of non-proteinogenic amino acids in the matrix of complex non-terrestrial samples. The procedure has proven to be highly sensitive and shows a wide linearity range with 0.005-3pmol detection limits for quantitative determinations. The developed procedure was tested on a sample of the Murchison meteorite, for which obtained chromatograms show excellent peak resolution, minimal co-elution and peak overlap. We conclude that comprehensive two dimensional chromatography, in combination with the optimized derivatization method is a highly suitable technique for the analysis of samples with very limited quantities and containing potentially prebiotic molecules, such as interstellar ice analogs and meteorites.

References

Dec 1, 1971·Chromatographic Reviews·V V Brazhnikov, L M Mukhin
Apr 20, 2005·Analytical and Bioanalytical Chemistry·Renuka MayadunnePhilip J Marriott
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Philippe Schmitt-KopplinNorbert Hertkorn
May 3, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Volker Schurig
Aug 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michael P CallahanJason P Dworkin
Jul 28, 2012·Journal of Chromatography. a·István IliszAntal Péter
Sep 12, 2012·Angewandte Chemie·Cornelia Meinert, Uwe J Meierhenrich

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Citations

Mar 22, 2018·Critical Reviews in Analytical Chemistry·Abdalla Ahmed Elbashir, Hassan Y Aboul-Enein
Apr 30, 2021·Analytical Methods : Advancing Methods and Applications·Lamei YangWeili Wei
May 11, 2021·Analytical Methods : Advancing Methods and Applications·Qianqian ZhaoYong Kong
Mar 21, 2019·ACS Earth & Space Chemistry·José C AponteJason P Dworkin

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