Quantitative evaluation of catecholamine enzymes gene expression in adrenal medulla and sympathetic Ganglia of stressed rats

Annals of the New York Academy of Sciences
R KvetnanskyOlga Krizanova

Abstract

Stress-induced changes in mRNA levels of tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH), and phenylethanolamine N-methyltransferase (PNMT) have been expressed as relative arbitrary units compared with a control group. The aim of this study was to quantify basal and stress-induced levels of TH, DBH, and PNMT mRNAs in rat adrenal medulla (AM) and stellate ganglia (SG) by the RT-competitive PCR method using corresponding competitors of known concentration. In rats stressed by immobilization (IMO) once for 2 h, the concentration of mRNAs was determined in various intervals after the end of stress stimulus. In SG, the basal concentration of TH mRNA was 0.017 amol/ng of total RNA, which is approximately 30 times lower than in the AM (0.460 amol/ng RNA). The basal concentration of DBH mRNA in SG was 2.60 amol/ng of total RNA, which is about 150 times more than TH mRNA in SG but only two times less than DBH mRNA in the AM in which PNMT mRNA is present in the highest concentration. After a single 2-h IMO, the peak elevation of TH and DBH mRNA concentration in SG occurred 24 h after the termination of stress stimulus, when their AM mRNA concentrations were already at control values. Presence of PNMT mRNA levels in the SG, of ...Continue Reading

References

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Citations

Jun 18, 2011·European Journal of Applied Physiology·Ljubica GavrilovicSladjana Dronjak
Mar 10, 2012·Cellular and Molecular Neurobiology·R KvetnanskyP Vargovic
Aug 29, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Benedek ErdosNihal Tümer
Jul 27, 2011·Brain, Behavior, and Immunity·Glaucie J AlvesJoão Palermo-Neto
Jan 6, 2009·Annals of the New York Academy of Sciences·Richard KvetnanskýLucia Kubovcakova
Jun 1, 2013·Autonomic Neuroscience : Basic & Clinical·Larisa BobrovskayaEugene Nalivaiko
Jun 2, 2009·Autonomic Neuroscience : Basic & Clinical·Ljubica GavrilovicSladjana Dronjak
Aug 2, 2013·Anais Da Academia Brasileira De Ciências·Ljubica GavrilovićSladjana Dronjak
Jun 21, 2006·Journal of Psychopharmacology·Murray EslerGavin Lambert
Apr 4, 2009·Physiological Reviews·Richard KvetnanskyMiklos Palkovits
Mar 31, 2017·American Journal of Physiology. Cell Physiology·Yedan GaiSheng Cui
Jan 18, 2019·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Anna VavřínováJosef Zicha
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Swati Sahoo, Brijesh S

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