Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo.

Clinical Ophthalmology
Masamichi Fukuda, Hiroshi Sasaki

Abstract

We attempted to develop a device for measuring electrical corneal resistance (CR) using corneal contact lens electrodes to quantitatively evaluate corneal injury in vivo. In the present study, full-thickness detachment of the corneal epithelium was induced by n-heptanol, and the feasibility of the quantitative evaluation of this injury by corneal contact lens electrodes was evaluated in vivo. The central area of an albino rabbit's cornea was exposed to a filter paper pre-immersed in n-heptanol for 1 minute to induce injury of the corneal epithelium. After induction of injury, the electrical CR was measured and the percentage of CR (%CR) was calculated. Fluorescein solution (3 μL) was applied to the wound/affected area of the corneal epithelium for photography with a slit-lamp biomicroscope. The wound/affected area was measured using an image analysis system. The correlation between the %CR and the wound/affected area was analyzed. As the size of the wound/affected area of the corneal epithelium increased, the %CR decreased after corneal epithelium detachment. Thus, a close correlation was found between the area of corneal epithelium detachment and the %CR. The corneal resistance device that we developed was capable of quantitat...Continue Reading

Citations

Mar 22, 2013·Clinical Ophthalmology·Masamichi FukudaHiroshi Sasaki
May 21, 2014·Sensors·Anton GuimeràRosa Villa
Oct 16, 2015·The Ocular Surface·Masafumi UematsuTakashi Kitaoka
Aug 21, 2015·Journal of Pharmacological and Toxicological Methods·Masafumi UematsuTakashi Kitaoka
Jul 18, 2015·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Masamichi Fukuda, Hiroshi Sasaki
Jul 24, 2013·Experimental Eye Research·Keith MooreJay D Potts
Oct 11, 2014·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Masamichi Fukuda, Hiroshi Sasaki
May 18, 2016·European Journal of Pharmacology·Masamichi FukudaHiroshi Sasaki
May 27, 2016·Cornea·Masamichi Fukuda, Hiroshi Sasaki
Jan 14, 2021·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Nobuo TakedaKazuhisa Sugiyama

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