Quantitative evaluation of lysozyme- and CD68-positive Kupffer cells in diethylnitrosamine-induced hepatocellular carcinomas in monkeys

Carcinogenesis
K LapisU P Thorgeirsson

Abstract

Quantitative analysis of lysozyme- and CD68-positive Kupffer cells was carried out in connection with diethylnitrosamine-induced hepatocarcinogenesis in non-human primates. The number of Kupffer cells/mm2 was determined in 28 cases of hepatocellular carcinoma (HCC) and seven age-matched controls. The Kupffer cell counts (mean +/-SEM) gradually decreased in the following order, irrespective of the histochemical markers (lysozyme or CD 68) used: healthy control liver (101.7 +/- 13.5 and 103.2 +/- 11.9 respectively), non-cirrhotic and non-neoplastic host liver (54.3 +/- 13.6 and 50.5 +/- 15.4), cirrhotic host liver (26.2 +/- 8.2 and 27.2 +/- 3.3), HCC tissue (20.7 +/- 4.4 and 19.3 +/- 4.1) and metastatic foci in the lung (9.8 +/- 1.8 and 9.7 +/- 2.8). The difference between the normal liver and the non-neoplastic, non-cirrhotic portions of the HCC-bearing liver was significant (P < 0.05). A highly significant difference was found between the number of Kupffer cells found in healthy control or non-neoplastic liver and those found in HCC nodules (P < 0.0001 and P < 0.0005 respectively). The results obtained by hematoxylin and eosin staining and lysozyme/CD68 immunohistochemistry were highly similar, indicating that this decrease was...Continue Reading

Citations

Apr 27, 2001·Cornea·M C KenneyD J Brown
Aug 2, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Bin ShiLaura Sepp-Lorenzino
Oct 23, 2008·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Shozo TakayamaRichard H Adamson
Oct 12, 2013·Journal of Digestive Diseases·Xiao Ling KuaiQing Zheng
Oct 27, 2020·Frontiers in Immunology·Xia WuIan N Crispe

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