Quantitative expression analyses of candidates for alternative anion conductance in cystic fibrosis mouse models

Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society
Josephine BraunAchim D Gruber

Abstract

Diversity of cystic fibrosis (CF) phenotype in patients with the same CFTR-mutation raised the hypothesis that other factors modulate the phenotype including "alternative" calcium-activated anion currents (CaCC). This study compares the mRNA expression levels of candidate CaCC mediators in CF mouse models with wild type controls. mBEST1, mBEST2, mCLC-3B, mCLC-4, mTTYH3, mTMEM16A, mTMEM16F, mTMEM16K, mCLCA1 to -6 and SLC26A9 mRNA were quantified in CF-relevant tissues in cftr(tm1Cam) and cftr(TgH(neoim)Hgu) mice and controls using real-time RT-qPCR. No consistent differences were observed except for mTTYH3 which was significantly down-regulated throughout the intestinal tract of cftr(tm1Cam) mice. Down-regulation of mTTYH3 may point towards its involvement in the complex CF pathology. However, the markedly reduced expression argues against a direct compensatory action as an alternative anion conductance. If any of the other candidates plays a role as modulator, factors other than transcriptional regulation and mRNA stability may be involved.

References

Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·L L ClarkeR C Boucher
May 1, 1993·Nature Genetics·R RatcliffW H Colledge
Aug 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J R DorinD J Porteous
Feb 15, 2002·Pflügers Archiv : European journal of physiology·K GyömöreyC E Bear
May 23, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ina Leverkoehne, Achim D Gruber
Mar 11, 2004·The Journal of Biological Chemistry·Makoto Suzuki, Atsuko Mizuno
Jul 9, 2004·Human Molecular Genetics·Lynn M UlatowskiMitchell L Drumm
Oct 19, 2004·Human Genetics·Margit RitzkaBurkhard Tümmler
May 28, 2005·The Journal of Biological Chemistry·Adele GibsonNicola Thompson
Jul 29, 2005·Histochemistry and Cell Biology·Dorthe von SmolinskiAchim D Gruber
Nov 30, 2005·Cellular and Molecular Life Sciences : CMLS·M SuzukiT Iwamoto
Feb 21, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Melanie K BotheAchim D Gruber
Apr 11, 2008·Physiology·Michael R DorwartShmuel Muallem
Jul 26, 2008·Biochimica Et Biophysica Acta·René Barro-SoriaKarl Kunzelmann
Sep 23, 2008·Cell·Björn Christian SchroederLily Yeh Jan
Sep 25, 2008·Current Opinion in Pulmonary Medicine·Joseph M Collaco, Garry R Cutting
Apr 14, 2009·The Journal of Biological Chemistry·Jason R RockBarbara R Grubb
Oct 16, 2009·Annual Review of Physiology·Charity DuranH Criss Hartzell
Dec 17, 2009·Histochemistry and Cell Biology·Josephine BraunAchim D Gruber

❮ Previous
Next ❯

Citations

Aug 3, 2011·Journal of Cellular Physiology·Jiraporn OusingsawatKarl Kunzelmann
Nov 28, 2015·Mediators of Inflammation·Monica Sala-RabanalTom J Brett
Mar 1, 2012·American Journal of Respiratory Cell and Molecular Biology·Lars MundhenkAchim D Gruber
Jan 17, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alicia K OlivierDavid K Meyerholz
Jul 16, 2021·Frontiers in Molecular Neuroscience·Rithvik R NalamalapuMargaret S Saha

❮ Previous
Next ❯

Related Concepts

Related Feeds

CFTR Mutant Structural Therapy

Over 1700 different mutations in the CFTR genes have been shown to cause cystic fibrosis. Here is the latest research on structural therapy for CFTR mutants.