PMID: 11933265Apr 5, 2002Paper

Quantitative FISH analysis on interphase nuclei may improve diagnosis of DNA diploid breast cancers

Diagnostic Cytopathology
Khuong TruongBernard Malfoy

Abstract

The detection of DNA aneuploid cells using flow cytometry is an indication for the presence of tumor cells, but when DNA diploid cells are found in 25-33% of the cases, the diagnostic and prognostic significance of DNA ploidy is more limited. We analyzed interphase nuclei after in situ hybridization and using image cytometry on 50 breast tumors with diploid DNA content to investigate whether early chromosome rearrangements were detectable and if their occurrence was clinically significant. Imbalances between the two arms of chromosome 1 were found in 55% of the cases and values ranged from 1.5-3.0. Comparison with histological data showed that Grade I tumors mainly have imbalances (67%) and that Grade III tumors were mainly without the imbalance (67%), whereas Grade II tumors were intermediate (50% imbalance). These data suggest that the diagnosis of DNA diploid cases may be improved by using interphase FISH. In addition, the data also indicates that early breast tumors may have different genetic origins, which is important in the comprehension of tumor malignancy in early stages, especially for preinvasive lesions.

References

Nov 1, 1991·Breast Cancer Research and Treatment·B DutrillauxM Prieur
May 1, 1993·Genes, Chromosomes & Cancer·N Kokalj-VokacB Dutrillaux
Dec 1, 1995·Genes, Chromosomes & Cancer·I Bièche, R Lidereau
Nov 21, 1998·Genes, Chromosomes & Cancer·D FlagielloB Dutrillaux
Feb 17, 2000·Journal of the National Cancer Institute·F M WaldmanB M Ljung
Mar 29, 2001·The Journal of Molecular Diagnostics : JMD·K TruongB Malfoy

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Citations

Jul 9, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Genta AmakawaKohsuke Sasaki
Jun 17, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Toshima Z ParrisKhalil Helou

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