Quantitative fluorescence imaging of mitochondria in body wall muscles of Caenorhabditis elegans under hyperglycemic conditions using a microfluidic chip.

Integrative Biology : Quantitative Biosciences From Nano to Macro
Samuel SofelaYong-Ak Song

Abstract

Type 2 diabetes is the most common metabolic disease, and insulin resistance plays a role in the pathogenesis of the disease. Because completely functional mitochondria are necessary to obtain glucose-stimulated insulin from pancreatic beta cells, dysfunction of mitochondrial oxidative pathway could be involved in the development of diabetes. As a simple animal model, Caenorhabditis elegans renders itself to investigate such metabolic mechanisms because it possesses insulin/insulin-like growth factor-1 signaling pathway similar to that in humans. Currently, the widely spread agarose pad-based immobilization technique for fluorescence imaging of the mitochondria in C. elegans is laborious, batchwise, and does not allow for facile handling of the worm. To overcome these technical challenges, we have developed a single-channel microfluidic device that can trap a C. elegans and allow to image the mitochondria in body wall muscles accurately and in higher throughput than the traditional approach. In specific, our microfluidic device took advantage of the proprioception of the worm to rotate its body in a microfluidic channel with an aspect ratio above one to gain more space for its undulation motion that was favorable for quantitati...Continue Reading

References

Sep 10, 2004·American Journal of Physiology. Cell Physiology·Paul S BrookesShey-Shing Sheu
Dec 17, 2004·Nature·Mehmet Fatih YanikAdela Ben-Yakar
Apr 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kevin R ShortK Sreekumaran Nair
Nov 14, 2006·Nucleic Acids Research·Lisa R GirardMartin Chalfie
Oct 26, 2007·Lab on a Chip·S Elizabeth HulmeGeorge M Whitesides
Nov 22, 2007·Aging Cell·Béatrice ChabiDavid A Hood
Feb 12, 2009·Lab on a Chip·Trushal Vijaykumar ChokshiNikos Chronis
Aug 14, 2009·Diabetes·Andreas SchlottererMichael Morcos
Dec 15, 2010·Biotechnology Journal·Maria Markaki, Nektarios Tavernarakis
Mar 9, 2012·Advances in Experimental Medicine and Biology·Philip NewsholmeMaurico Krause
May 26, 2012·Frontiers in Physiology·Nicole Shangming Hou, Stefan Taubert
Apr 13, 2013·Cold Spring Harbor Perspectives in Biology·Paolo Bernardi, Paolo Bonaldo
Jun 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Wouter De HaesLiesbet Temmerman
Oct 14, 2014·Frontiers in Aging Neuroscience·Russell T Hepple
Mar 15, 2015·Journal of Applied Physiology·Martin PicardBasil J Petrof
Apr 22, 2015·Nature·Konstantinos PalikarasNektarios Tavernarakis
Dec 15, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Guoli ZhuJianhua Qin
Mar 16, 2016·IEEE Transactions on Medical Imaging·Nima Tajbakhsh Jianming Liang
Oct 22, 2016·Frontiers in Physiology·Gilles Gouspillou, Russell T Hepple
Jan 7, 2017·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·G GopakumarGorthi R K Sai Subrahmanyam
Feb 23, 2018·Nature·Sarah Webb
Jun 13, 2018·Annual Review of Analytical Chemistry·Farhan Kamili, Hang Lu
Aug 18, 2018·Integrative Biology : Quantitative Biosciences From Nano to Macro·Ivan de Carlos CáceresHang Lu
Apr 12, 2019·Frontiers in Genetics·Binhua TangAsif Khateeb
Dec 18, 2019·PloS One·Jonathan Alcántar-FernándezJuan Miranda-Ríos
Feb 1, 2018·Nature·Sarah Webb

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Related Papers

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Muniesh Muthaiyan Shanmugam, Tuhin Subhra Santra
BioRxiv : the Preprint Server for Biology
J. ShiversClifford P. Brangwynne
© 2022 Meta ULC. All rights reserved