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Quantitative fractionation of alkaline phosphatase isoenzymes according to their thermostability

Clinical Chemistry

Jan 1, 1976

C J Petitclerc

PMID: 1158

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Abstract

Continuous monitoring of heat denaturation of a mixture of alkaline phosphatase isoenzymes at 60 degrees C and pH 7.5 permits the simultaneous direct identification and quantitation of three isoenzymes: the placental isoenzyme, the L-phenylalanine-sensitive intestinal isoenzyme, and the...read more

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Skeletal Bone
Orthophosphate
Duodenum
Gamma-glutamyl transferase
Alloenzymes
Hydrogen-Ion Concentration
Alkaline Phosphatase
Liver Diseases
Protein Denaturation
Blood
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Quantitative fractionation of alkaline phosphatase isoenzymes according to their thermostability

Clinical Chemistry

Jan 1, 1976

C J Petitclerc

PMID: 1158

DOI:

Abstract

Continuous monitoring of heat denaturation of a mixture of alkaline phosphatase isoenzymes at 60 degrees C and pH 7.5 permits the simultaneous direct identification and quantitation of three isoenzymes: the placental isoenzyme, the L-phenylalanine-sensitive intestinal isoenzyme, and the...read more

Mentioned in this Paper

Skeletal Bone
Orthophosphate
Duodenum
Gamma-glutamyl transferase
Alloenzymes

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