Quantitative gas chromatography-mass spectrometry isomer-specific measurement of hydroxy fatty acids in biological samples and food as a marker of lipid peroxidation

Analytical Biochemistry
R WilsonR A Riemersma

Abstract

We have developed a capillary gas chromatography-mass spectrometry method for the quantitative analysis of individual positional isomers of monohydroxy fatty acids derived from linoleic, arachidonic, eicosapentaenoic, or docosahexaenoic acid. Peroxidation of a particular polyunsaturated fatty acid results already in a complex mixture of positional isomers of hydroperoxy and hydroxy fatty acids. Catalytic hydrogenation of lipid extracts produces stable saturated hydroxy lipids from the complex mixtures typical of oxidized biological samples, simultaneously simplifying the analytical problem and eliminating oxidation artifacts. After saponification and methylation, monohydroxy fatty acid methyl esters are purified by solid-phase extraction and partially resolved using a CP Sil 19 column following on-column derivatization of the hydroxy groups with tetramethylammonium hydroxide. The resulting methoxy fatty acid methyl esters are subjected to electron impact mass spectroscopy. Two characteristic ions are produced for each positional isomer. Quantitative measurements were achieved by using odd chain C17 and C19 monohydroxy fatty acids as internal standards. The limit of detection of individual hydroxy fatty acid isomers is dependent...Continue Reading

References

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Jan 1, 1986·Analytical Biochemistry·H HughesJ R Mitchell
Jan 1, 1993·Free Radical Research Communications·J M Gutteridge

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Citations

Mar 16, 2002·European Journal of Clinical Investigation·R WilsonR A Riemersma
Jan 4, 2015·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Robert C BlockShaker A Mousa
Feb 6, 2017·Journal of Agricultural and Food Chemistry·Christine E RichardsonAmeer Y Taha
Jul 16, 2019·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Nahmah Kim-CampbellHülya Bayír
Jul 31, 2013·Journal of Separation Science·Muhammed AlzweiriMohammad A Khanfar
Jul 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Wei Xia, Suzanne M Budge
Aug 29, 2003·Organic Letters·Max J CryleJames J De Voss
Mar 25, 2005·The Journal of Organic Chemistry·Max J CryleJames J De Voss
Feb 14, 2002·Free Radical Biology & Medicine·Robert WilsonRudolph A Riemersma
Aug 15, 2000·Journal of Chromatography. a·V Ruiz-Gutiérrez, M C Pérez-Camino

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