Quantitative Glycomic Analysis by Mass-Defect-Based Dimethyl Pyrimidinyl Ornithine (DiPyrO) Tags and High-Resolution Mass Spectrometry

Analytical Chemistry
Bingming ChenLingjun Li

Abstract

We recently developed a novel amine-reactive mass-defect-based chemical tag, dimethyl pyrimidinyl ornithine (DiPyrO), for quantitative proteomic analysis at the MS1 level. In this work, we further extend the application of the DiPyrO tag, which provides amine group reactivity, optical detection capability, and improved electrospray sensitivity, to quantify N-linked glycans enzymatically released from glycoproteins in the glycosylamine form. Duplex DiPyrO tags that differ in mass by 45.3 mDa were used to label the glycosylamine moieties of freshly released N-glycosylamines from glycoprotein standards and human serum proteins. We demonstrate that both MALDI-LTQ-Orbitrap and nano-HILIC LC/MS/MS Fusion Lumos Orbitrap platforms are capable of resolving the singly or multiply charged N-glycans labeled with mass-defect DiPyrO tags. Dynamic range of quantification, based on MS1 peak intensities, was evaluated across 2 orders of magnitude. With optimized N-glycan release conditions, glycosylamine labeling conditions, and MS acquisition parameters, the N-glycan profiles and abundances in human serum proteins of cancer patients before and after chemotherapy were compared. Moreover, this study also opens a door for using well-developed ami...Continue Reading

References

Mar 28, 1996·Chemical Reviews·Raymond A. Dwek
Nov 16, 2007·Journal of Cellular Biochemistry·Pamela A NortonTimothy M Block
Dec 1, 2007·Journal of Proteome Research·James A AtwoodRon Orlando
Sep 25, 2009·Current Opinion in Chemical Biology·Hyun Joo AnCarlito B Lebrilla
Mar 13, 2010·Analytical and Bioanalytical Chemistry·L R RuhaakM Wuhrer
Sep 13, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Takehiro OyamaShigeo Suzuki
Mar 30, 2012·Analytical Chemistry·Hannes HahneBernhard Kuster
Jun 23, 2012·Nature Reviews. Molecular Cell Biology·Kelley W MoremenAlison V Nairn
Jun 26, 2012·Rapid Communications in Mass Spectrometry : RCM·Mikhail V GorshkovYury O Tsybin
Feb 26, 2013·Nature Methods·Alexander S HebertJoshua J Coon
Mar 28, 2013·Chemical Reviews·William R AlleyMilos V Novotny
Jul 19, 2013·Journal of the American Society for Mass Spectrometry·S Hunter WalkerDavid C Muddiman
Jul 31, 2013·Analytical Chemistry·Shuang YangHui Zhang
Apr 30, 2014·The Analyst·S DefausR Gutiérrez-Gallego
Jun 19, 2014·Molecular & Cellular Proteomics : MCP·Anna E MerrillJoshua J Coon
Dec 1, 2015·Scientific Reports·Shuang YangShuwei Li

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Citations

Jan 10, 2020·Expert Review of Proteomics·Wei-Qian CaoPeng-Yuan Yang
Feb 11, 2021·Mass Spectrometry Reviews·Milani W PatabandigeHeather Desaire
Jun 25, 2020·Molecular & Cellular Proteomics : MCP·Daniel G Delafield, Lingjun Li
May 14, 2020·Analytical Chemistry·Dustin C FrostLingjun Li
Nov 6, 2018·Analytical Chemistry·Tianjiao HuangJames L Edwards
Sep 11, 2021·Electrophoresis·Kaitlyn B DonohooYehia Mechref

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