Quantitative histopathological analysis of cervical intra-epithelial neoplasia sections: methodological issues

Cellular Oncology : the Official Journal of the International Society for Cellular Oncology
Martial GuillaudCalum MacAulay

Abstract

As part a Program Project to evaluate emerging optical technologies for cervical neoplasia, our group is performing quantitative histopathological analysis of biopsies from 1,800 patients. Several methodological issues have arisen with respect to this analysis: (1) Finding the most efficient way to compensate for staining intensity variation with out losing diagnostic information; (2) Assessing the inter- and intra-observer variability of the semi-interactive data collection; and (3) the use of non-overlapping cells from the intermediate layer only. Non-overlapping quantitatively stained nuclei were selected from 280 samples with histopathological characteristics of normal (199), koilocytosis (37), CIN 1 (18), CIN 2 (10) and CIN 3 (16). Linear discriminant analysis was used to assess the diagnostic information in three different feature sets to evaluate and compare staining intensity normalization methods. Selected feature values and summary scores were used to evaluate intra- and inter-observer variability. The features normalized by the internal subset of the imaged cells had the same discriminatory power as those normalized by the control cells and by both normalization methods seem to have additional discriminatory power ov...Continue Reading

Citations

Feb 11, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mohammad Shifat-E-RabbiGustavo K Rohde

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