PMID: 3401283Jun 1, 1988Paper

Quantitative HPLC analysis of human plaque proteins in coronary and thoracic aorta arteries

Atherosclerosis
R P LiburdyB P Mindich

Abstract

Quantitative HPLC analysis of saline-soluble proteins obtained from human coronary and thoracic aorta plaque and from whole internal mammary artery were performed. Protein extracts were characterized by anion exchange and reverse-phase HPLC and the integrated chromatographs revealed significant differences in both peak retention times and areas for protein species from coronary artery compared to thoracic aorta artery plaque. Coronary artery plaque proteins possessed a high degree of cationic charge and polarity compared to those present in thoracic aorta plaque and normal mammary artery. This suggests that specific protein markers may be expressed in plaque of different anatomical origin, and that the processing of protein may be distinct to plaque sites. In contrast, characterization of molecular weight by gel electrophoresis resolved no major differences between plaque types. These findings indicate that proteins in human plaque lesions of different anatomical origin can be resolved by HPLC methodology and that they exhibit different charge and polarity. Such an HPLC approach may prove useful in the quantitative identification and ultimate isolation of specific protein markers present in plaque during atherogenesis, and in t...Continue Reading

References

Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G E KatzensteinC R Middaugh
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N IshiokaF W Putnam
Jul 15, 1982·Analytical Biochemistry·J D PearsonF E Regnier
Oct 19, 1984·Science·T F Budinger, P C Lauterbur

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Citations

Mar 10, 2001·The Annals of Thoracic Surgery·R P Scott

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