Quantitative Hybrid Cardiac [18 F]FDG-PET-MRI Images for Assessment of Cardiac Repair by Preconditioned Cardiosphere-Derived Cells

Molecular Therapy. Methods & Clinical Development
Johannes WinklerMariann Gyöngyösi

Abstract

Cardiosphere-derived cells (CDCs) are progenitor cells derived from heart tissue and have shown promising results in preclinical models. APOSEC, the secretome of irradiated peripheral blood mononuclear cells, has decreased infarct size in acute and chronic experimental myocardial infarction (MI). We enhanced the effect of CDCs with APOSEC preconditioning (apoCDC) and investigated the reparative effect in a translational pig model of reperfused MI. Supernatants of CDCs, assessed by proteomic analysis, revealed reduced production of extracellular matrix proteins after in vitro APOSEC preconditioning. In a porcine model of catheter-based reperfused anterior acute MI (AMI), CDCs with (apoCDC, n = 8) or without APOSEC preconditioning (CDC, n = 6) were infused intracoronary, 15 min after the start of reperfusion. Untreated AMI animals (n = 7) and sham procedures (n = 5) functioned as controls. 2-deoxy-2-(18 F)-fluoro-D-glucose-positron emission tomography-magnetic resonance imaging ([18F]FDG-PET-MRI), with late enhancement after 1 month, showed reduced scar volume and lower transmurality of the infarcted area in CDC and apoCDC compared to AMI controls. Segmental quantitative PET images displayed indicated more residual viability in a...Continue Reading

References

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Citations

Feb 13, 2021·Expert Opinion on Biological Therapy·Michail E KlontzasApostolos H Karantanas
Mar 27, 2021·Molecular Therapy. Methods & Clinical Development·Maria LaggnerHendrik Jan Ankersmit

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Methods Mentioned

BETA
biopsies
ELISA

Software Mentioned

clusterProfiler
SPSS
Segment
R
MACSQuant
Slicer
APOSEC
limma
3D

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