Quantitative image analysis of intra-tumoral bFGF level as a molecular marker of paclitaxel resistance.

Journal of Translational Medicine
Colin T WalshJessie L-S Au

Abstract

The role of basic fibroblast growth factor (bFGF) in chemoresistance is controversial; some studies showed a relationship between higher bFGF level and chemoresistance while other studies showed the opposite finding. The goal of the present study was to quantify bFGF levels in archived tumor tissues, and to determine its relationship with chemosensitivity. We established an image analysis-based method to quantify and convert the immunostaining intensity of intra-tumor bFGF to concentrations; this was accomplished by generating standard curves using human xenograft tumors as the renewable tissue source for simultaneous image analysis and ELISA. The relationships between bFGF concentrations and tumor chemosensitivity of patient tumors (n = 87) to paclitaxel were evaluated using linear regression analysis. The image analysis results were compared to our previous results obtained using a conventional, semi-quantitative visual scoring method. While both analyses indicated an inverse relationship between bFGF level and tumor sensitivity to paclitaxel, the image analysis method, by providing bFGF levels in individual tumors and therefore more data points (87 numerical values as opposed to four groups of staining intensities), further ...Continue Reading

References

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Citations

Jul 7, 2009·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Vagner BernardoUlysses Lins
Jan 15, 2014·Journal of Chemical Neuroanatomy·Geraldine K E GoebrechtJ Matthew Kittelberger
May 28, 2010·Journal of Cellular Biochemistry·Miao WangYu-Xin Li
Mar 31, 2016·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Abbas ShirinifardAndrás Sablauer
Nov 6, 2013·International Journal of Molecular Medicine·Wu LuoXiaoping Wu

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Methods Mentioned

BETA
xenografts
ELISA
xenograft

Software Mentioned

Optimas
SAS

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