Quantitative immunocytochemistry of glia in the cerebellar cortex of old ethanol-fed rats

Alcohol
Cynthia A Dlugos, R Pentney

Abstract

It is clear from results of studies in this laboratory that chronic ethanol consumption causes regression of the extensive Purkinje neuron (PN) dendritic arbor. There are, however, a paucity of studies on the effects of chronic ethanol consumption on glia cells that reside in the molecular layer of the cerebellar cortex with PN dendrites. The purpose of the present study was to investigate the possibility that chronic ethanol consumption in old F344 rats results in gliosis within the molecular layer of the cerebellar cortex. Ten 12-month-old, male, F344 rats received a liquid diet containing 35% ethanol for 40 weeks. Pair-fed controls (n=10) received a liquid diet in which maltose dextrins were substituted for ethanol. Chow-fed rats (n=10) served as controls for age. At the end of the treatment period, rats were euthanized and perfused through the aorta, and cerebella were prepared for immunocytochemistry. Free floating sections were stained with (1) glial fibrillary acidic protein antibody for labeling of Bergmann glial cells and fibers, (2) OX-42 antibody for labeling of microglia, and (3) 0.5% cresyl violet for estimates of molecular layer volume. Results indicate that the densities of Bergmann glial cell processes and micro...Continue Reading

References

Nov 1, 1991·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T MoriokaW J Streit
Oct 1, 1991·Neuropathology and Applied Neurobiology·D A CrooksS Evans
Dec 1, 1991·Alcoholism, Clinical and Experimental Research·R J Pentney, L J Quackenbush
Jan 1, 1991·Glia·M E HattenC A Mason
Dec 1, 1990·Alcoholism, Clinical and Experimental Research·R J Pentney, L J Quackenbush
Jan 1, 1988·Glia·W J StreitG W Kreutzberg
Jun 1, 1987·Alcoholism, Clinical and Experimental Research·M A TavaresA Cadete-Leite
Jan 1, 1970·Acta Anatomica·W A Merz, R K Schenk
Nov 1, 1994·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A K ShettyD E Phillips
May 1, 1994·Journal of Neuropathology and Experimental Neurology·M D Norenberg
Sep 1, 1993·Neurobiology of Aging·N R NicholsC E Finch
Mar 1, 1997·Neurotoxicology and Teratology·A HolowniaJ F Ménez
Feb 24, 1999·Alcoholism, Clinical and Experimental Research·L Korbo
Nov 24, 1999·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·J M LongD K Ingram
Feb 24, 2000·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·R J Pentney, C A Dlugos

❮ Previous
Next ❯

Citations

Oct 23, 2008·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Fulton T Crews, Kim Nixon
Jan 22, 2009·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Roberta J WardPhilippe de Witte
Jul 26, 2013·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Linda L CassidyCynthia A Dlugos
May 25, 2013·Journal of Neuroinflammation·Cynthia J M KanePaul D Drew
Apr 9, 2010·Alcoholism, Clinical and Experimental Research·Cynthia A Dlugos, Richard A Rabin
Mar 31, 2007·The Journal of Comparative Neurology·Cynthia A Dlugos, Richard A Rabin
Apr 21, 2017·Frontiers in Cellular Neuroscience·Juan A OrellanaRodrigo A Quintanilla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.