Quantitative immunohistochemical analysis of myeloid cell marker expression in human cortex captures microglia heterogeneity with anatomical context.

Scientific Reports
Molly E V SwansonMaurice A Curtis

Abstract

Current immunohistochemical methods of studying microglia in the post-mortem human brain do not capture the heterogeneity of microglial function in response to damage and disease. We therefore investigated the expression of eight myeloid cell proteins associated with changes in function alongside Iba1. To study the myeloid cells we used immunohistochemistry on post-mortem human middle temporal gyrus sections from neurologically normal individuals. First we investigated co-labelling between the classical 'activation' marker, HLA-DR and each of the other markers of interest. Significant co-labelling between HLA-DR with CD206, CD32, CD163, or L-Ferritin was observed, although complete overlap of expression of HLA-DR with aforementioned markers was not observed. A qualitative assessment also demonstrated that perivascular macrophages expressed higher levels of the markers of interest we investigated than microglia, suggesting perivascular macrophages show a more phagocytic and antigen presentation state in the human brain. To determine whether the markers of interest were expressed in different functional states, the immunoreactivity for each marker was qualitatively assessed on microglial morphologies. Degenerating marker, L-Ferri...Continue Reading

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Citations

Jul 22, 2021·Neuro-oncology Advances·Zoe WoolfMichael Dragunow
Jul 21, 2021·Stroke; a Journal of Cerebral Circulation·Anan ShtayaAtticus H Hainsworth
Sep 23, 2021·Biological Reviews of the Cambridge Philosophical Society·Marcus Augusto-OliveiraAlexei Verkhratsky
Nov 9, 2021·Cell Biology International·Prateep AmonruttanapunSiriporn Chamniansawat

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