Quantitative immunolocalization of mu opioid receptors: regulation by naltrexone

Neuroscience
E M UnterwaldC J Evans

Abstract

The present study utilized a newly developed quantitative immunohistochemical assay to measure changes in mu opioid receptor abundance following chronic administration of the opioid receptor antagonist naltrexone. These data were compared with those obtained from mu receptor radioligand binding on adjacent tissue sections, in order to determine whether the characteristic antagonist-induced increase in radioligand binding is due to an increase in the total number of mu receptors and/or to an increase in the proportion of receptors that are in an active binding conformation in the absence of a change in the total number of receptors. Adult male Sprague-Dawley rats were administered naltrexone, 7-8 mg/kg per day, or saline continuously for seven days by osmotic minipumps, after which time their brains were processed for immunohistochemistry and receptor autoradiography on adjacent fresh frozen tissue sections. Semiquantitative immunohistochemistry was performed using a radiolabelled secondary antibody for autoradiographic determination and a set of radioactive standards. Results demonstrate an overall concordance between the distribution of mu opioid receptors as measured by the two different methods with a few exceptions. Followi...Continue Reading

References

Sep 15, 1978·European Journal of Pharmacology·R A Lahti, R J Collins
Jan 1, 1991·Life Sciences·S R Childers
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R A NorthM J Christie
Feb 1, 1985·Pharmacology, Biochemistry, and Behavior·S Schenk, E Nawiesniak
Aug 12, 1982·Brain Research·R S ZukinA R Gintzler
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A TempelR S Zukin
Dec 1, 1993·Trends in Neurosciences·T Reisine, G I Bell
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J B WangG R Uhl
Aug 9, 1996·The Journal of Biological Chemistry·D E KeithM von Zastrow
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·C SterniniN C Brecha
Nov 15, 1996·The Journal of Biological Chemistry·N TrapaidzeL A Devi

❮ Previous
Next ❯

Citations

Jun 29, 2004·Behavioural Brain Research·Carla Sanchis-SeguraCarlos M G Aragon
Feb 10, 2004·Brain Research. Developmental Brain Research·Eli M MrkusichDarren J Day
Sep 6, 2003·Pharmacology, Biochemistry, and Behavior·Vikram RajashekaraByron C Yoburn
Sep 1, 2008·Journal of Addiction Medicine·Ellen M Unterwald
Mar 24, 2005·Journal of Addictive Diseases·Joanne A ByarsMark S Gold
Jan 1, 2016·Journal of Neurochemistry·Sumita ChakrabartiAlan R Gintzler
Jul 23, 2003·British Journal of Pharmacology·Alexis BaileyIan Kitchen
Mar 26, 2003·The European Journal of Neuroscience·Heidi M B LesscherMirjam A F M Gerrits
Dec 21, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ulla E Petäjä-Repo, Jarkko J Lackman
Jul 8, 2008·Progress in Neurobiology·Ilana Roth-DeriGal Yadid
Jul 13, 2011·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Francesco LeriMary Jeanne Kreek
Sep 11, 1998·The Journal of Comparative Neurology·G Monteillet-AgiusC J Evans
Aug 4, 2018·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Ingrid A Binswanger, Jason M Glanz
Aug 22, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Raymond T BartusAnthony S Basile
Oct 17, 2020·Frontiers in Psychiatry·Christopher K Blazes, Jonathan D Morrow
Apr 20, 2021·Pharmacological Reports : PR·Mona KhoramjouySoraya Shahhosseini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.