PMID: 2115999Jan 1, 1990Paper

Quantitative in vitro assay for crustacean chromatophorotropins and other pigment cell agonists

Pigment Cell Research
A L BrittoL Josefsson

Abstract

An in vitro crustacean (freshwater shrimp, Macrobrachium potiuna) erythrophore bioassay for chromatophorotropins and other pigment cell agonists is described. The present assay is a quantitative method that determines the pigment responses with the aid of an ocular micrometer. The pigment granules within the erythrophores are dispersed out into the dendritic processes of the cells when the isolated carapace is placed in physiological solution. This bioassay provides, therefore, a method for measuring the response of the pigment cells to aggregating agents such as pigment concentrating hormone (PCH). This bioassay is sensitive to PCH at a concentration as low as 3 x 10(-12) M. Calcium ionophore A23187 mimics the actions of PCH, but, unlike the hormone, the ionophore-induced pigment aggregation is irreversible after physiological solution rinses. Therefore, chromatophorotropic activities of pigment dispersing agents, such as pigment dispersing hormones (PDH), can be determined on ionophore-treated erythrophores. The potencies of alpha-PDH and beta-PDH show a threefold difference (not significant). Because of its convenience and its ability to make an objective determination of the bidirectional pigment movements within erythropho...Continue Reading

References

Jul 19, 1976·Biochimica Et Biophysica Acta·P Fernlund
Nov 1, 1976·General and Comparative Endocrinology·J CarlsenL Josefsson
May 1, 1983·International Journal of Peptide and Protein Research·L Josefsson
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K R RaoM F Hintz

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Citations

Mar 20, 1998·Comparative Biochemistry and Physiology. Part A, Physiology·L E Nery, A M Castrucci
Apr 23, 2008·Pigment Cell & Melanoma Research·Marcelo Alves VargasLuiz Eduardo Maia Nery
Jun 26, 2009·Insect Biochemistry and Molecular Biology·Kiyoshi Hiruma, Lynn M Riddiford
Jan 26, 2007·Pigment Cell Research·Márcia Ribeiro, John Campbell McNamara
Aug 5, 2010·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Sarah Ribeiro MilogranaJohn Campbell McNamara
Aug 18, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Flavia Cristina GranatoLuiz Eduardo Maia Nery
Oct 26, 1999·The Journal of Experimental Zoology·L E NeryA M Castrucci
Dec 10, 2016·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Sarah Ribeiro MilogranaJohn Campbell McNamara

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