Quantitative liquid chromatographic-tandem mass spectrometric determination of orlistat in plasma with a quadrupole ion trap

Journal of Chromatography. B, Biomedical Sciences and Applications
R WieboldtJ Henion

Abstract

This report evaluates the use of a quadrupolar ion trap for quantitation in a bioanalytical laboratory. The evaluation was accomplished with the cross-validation of an LC-MS-MS quantitative method previously validated on a triple quadrupole mass spectrometer. The method was a multi-level determination of the anti-obesity drug, orlistat, in human plasma. The method has been refined previously on a triple quadrupole instrument to provide rapid sample throughput with robust reproducibility at sub-nanogram detection limits. Optimization of the method on the ion trap required improved chromatographic separation of orlistat from interfering plasma matrix components coextracted during the initial liquid-liquid extraction of plasma samples. The ion trap produces full-scan collision-induced dissociation mass spectra containing characteristic orlistat fragment ions that are useful for quantitation. Data collection on the ion trap required a precursor ion isolation width of 3.0 Da and optimal quantitative results were obtained when three fragment ions were monitored with a 1.8 Da window for each ion. Although a direct cross-validation between the ion trap and the tandem triple quadrupole mass spectrometer was not possible, quantitative re...Continue Reading

References

Nov 1, 1993·Journal of Pharmaceutical and Biomedical Analysis·J HenionA Mordehai
Mar 1, 1996·The American Journal of Clinical Nutrition·A M Wolf, G A Colditz
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Jan 1, 1997·Analytical Biochemistry·K R Jonscher, J R Yates

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Citations

Nov 24, 1999·Mass Spectrometry Reviews·M S Lee, E H Kerns
Jul 3, 2003·Mass Spectrometry Reviews·Gérard Hopfgartner, Emmanuel Bourgogne
Jan 17, 2004·International Journal of Pharmaceutics·P Chris de SmidtIñaki F Trocóniz
Apr 3, 2009·Journal of the American Society for Mass Spectrometry·Kevin P BatemanLester Taylor
Feb 3, 2007·Chemical & Pharmaceutical Bulletin·Effat SouriBettina Zadehvakili
Jan 20, 2007·AAPS PharmSciTech·Sunil K JainNarendra K Jain
Jan 1, 2001·Journal of Liposome Research·F J Belas, I A Blair
Sep 25, 2001·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R S PlumbR A Biddlecombe
Sep 5, 2003·Rapid Communications in Mass Spectrometry : RCM·Pearl K S BlayDietrich A Volmer

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