Quantitative MALDI-MS(n) analysis of cocaine in the autopsied brain of a human cocaine user employing a wide isolation window and internal standards.

Journal of the American Society for Mass Spectrometry
Richard F ReichRichard A Yost

Abstract

Detection of drugs in tissue typically requires extensive sample preparation in which the tissue is first homogenized, followed by drug extraction, before the extracts are finally analyzed by LC/MS. Directly analyzing drugs in intact tissue would eliminate any complications introduced by sample pretreatment. A matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS(n)) method as been developed for the quantification of cocaine present in postmortem brain tissue of a chronic human cocaine user. It is shown that tandem mass spectrometry (MS(2) and MS(3) increase selectivity, which is critical for differentiating analyte ions from background ions such as matrix clusters and endogenous compounds found in brain tissue. It is also shown that the use of internal standards corrects for signal variability during quantitative MALDI, which can be caused by inhomogeneous crystal formation, inconsistent sample preparation, and laser shot-to-shot variability. The MALDI-MS(n) method developed allows for a single MS(3) experiment that uses a wide isolation window to isolate both analyte and internal standard target ions. This method is shown to provide improved precision [approximately 10-20 times reduction in percent re...Continue Reading

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Citations

Sep 29, 2011·Journal of the American Society for Mass Spectrometry·M Lisa ManierRichard M Caprioli
Apr 23, 2011·Analytical Chemistry·Tiffany PortaGérard Hopfgartner
Dec 24, 2011·Bioanalysis·Stacey R Oppenheimer, Dieter M Drexler
Jan 28, 2016·Analytical Chemistry·Chad W ChumbleyRichard M Caprioli
Dec 10, 2014·Analytical and Bioanalytical Chemistry·Tiffany PortaGérard Hopfgartner
Aug 16, 2016·Bioanalysis·Andrea E SteuerThomas Kraemer
Sep 23, 2016·Journal of the American Society for Mass Spectrometry·Boone M PrenticeRichard M Caprioli
May 29, 2012·Rapid Communications in Mass Spectrometry : RCM·Nozomi TakaiHideo Saji
Jun 20, 2013·Expert Review of Proteomics·Patrick Neubert, Axel Walch
Jun 2, 2011·Journal of Mass Spectrometry : JMS·Fabian DeutskensRichard M Caprioli
Nov 4, 2020·Analytical and Bioanalytical Chemistry·Vilmos Kertesz, John F Cahill
Feb 4, 2021·Analytical and Bioanalytical Chemistry·Angus C GreyNicholas J Demarais
Jan 14, 2020·Analytical Chemistry·Vanessa Y RubioTimothy J Garrett
Dec 20, 2014·Analytical Chemistry·Anna NilssonPer E Andrén

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