Quantitative mapping of the orientation of fibroin beta-sheets in B. mori cocoon fibers by scanning transmission X-ray microscopy

Biomacromolecules
Daniel Hernández CruzAdam P Hitchcock

Abstract

The spatial distribution of the linear dichroic signal associated with aligned beta-sheets in a microtomed section of a Bombyx mori cocoon silk fiber was derived from scanning transmission X-ray microscopy (STXM). The intense C 1s --> pi(amide) peak at 288.25 eV was found to have negligible dichroic signal in transverse sections but a large dichroic signal in longitudinal sections. This is consistent with other measurements of the orientation of the aligned beta-sheets in silk fibers, in particular with those obtained by polarized Raman microspectroscopy to which our results are compared. When the dichroic signal strength is mapped at better than 100 nm spatial resolution, microscopic variations are found. Although the magnitude of the dichroic signal changes over a fine spatial scale, the direction of the maximum signal at any position does not change. We interpret the spatial variation of the intensity of the dichroic signal as a map of the quality of local orientation of the beta-sheets in the fiber. At sufficiently high magnification and resolution, this technique should image individual beta-sheet crystallites, although the present implementation does not achieve that. A map of the orientation parameter P(2) is derived. Th...Continue Reading

References

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Citations

Apr 7, 2007·The Review of Scientific Instruments·D Hernández-CruzM Pézolet
Aug 31, 2016·Topics in Current Chemistry·Loïc BertrandUwe Bergmann
Sep 13, 2008·Micron : the International Research and Review Journal for Microscopy·Adam P HitchcockGianluigi Botton
Nov 13, 2007·Micron : the International Research and Review Journal for Microscopy·Adam P HitchcockGianluigi Botton
Jan 2, 2015·Micron : the International Research and Review Journal for Microscopy·Andreas SpäthRainer H Fink
Feb 6, 2007·Biophysical Journal·Thierry LefèvreMichel Pézolet
Jul 20, 2017·Nature Communications·Ulyana ShimanovichTuomas P J Knowles
Jun 11, 2019·Physical Chemistry Chemical Physics : PCCP·L WeinhardtC Heske
Aug 9, 2017·Scientific Reports·Meguya RyuJunko Morikawa
Dec 7, 2018·Scientific Reports·Reo HondaJunko Morikawa
Mar 25, 2008·The Journal of Physical Chemistry. B·Yan ZubavichusMichael Zharnikov
Jun 15, 2007·The Journal of Physical Chemistry. B·Jacob Stewart-OrnsteinRobert E W Hancock
Jun 9, 2018·Analytical Chemistry·Corentin Le GuillouYann Le Brech
Aug 23, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaosong LiuF J Himpsel
Mar 14, 2007·Journal of the American Chemical Society·Marie-Eve RousseauMichel Pézolet
Aug 1, 2007·The Journal of Physical Chemistry. B·Yan ZubavichusMichael Zharnikov
Jun 15, 2006·Analytical Chemistry·Marian NavratilEdgar A Arriaga

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