Quantitative measure of small-subunit rRNA gene sequences of the kingdom korarchaeota

Applied and Environmental Microbiology
C F Brunk, N Eis


Comparative PCR amplification of small-subunit (SSU) rRNA gene (rDNA) sequences indicates substantial preferential PCR amplification of pJP27 sequences with korarchaeote-specific PCR primers. The coamplification of a modified SSU rDNA sequence can be used as an internal standard to determine the amount of a specific SSU rDNA sequence.


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