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Quantitative measurement for endothelial constitutive nitric oxide synthase in cultured human endothelial cells

Nitric Oxide : Biology and Chemistry

Jun 1, 1997

S AberleKatalin Kauser

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Abstract

Constitutively expressed endothelial nitric oxide synthase (ecNOS) produces nitric oxide (NO) from L-arginine and is important for the maintenance of cardiovascular homeostasis. We report the development of a capture ELISA which is specific for ecNOS. The assay detection limit is 0.5 ng...read more

Mentioned in this Paper

Enzyme-Linked Immunosorbent Assay
Down-Regulation
Arginine
Cricetus
Aorta
Assay
Nitric Oxide
NOS3 gene
Western Blot
NOS3 protein, human

Quantitative measurement for endothelial constitutive nitric oxide synthase in cultured human endothelial cells

Nitric Oxide : Biology and Chemistry

Jun 1, 1997

S AberleKatalin Kauser

PMID: 9704584

DOI: 10.1006/niox.1997.0129

Abstract

Constitutively expressed endothelial nitric oxide synthase (ecNOS) produces nitric oxide (NO) from L-arginine and is important for the maintenance of cardiovascular homeostasis. We report the development of a capture ELISA which is specific for ecNOS. The assay detection limit is 0.5 ng...read more

Mentioned in this Paper

Enzyme-Linked Immunosorbent Assay
Down-Regulation
Arginine
Cricetus
Aorta

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