Quantitative measurement of acetyl fentanyl and acetyl norfentanyl in human urine by LC-MS/MS

Analytical Chemistry
Amy L PattonJeffery H Moran

Abstract

Opioid abuse involving emerging opioid compounds is a growing public health problem, which was highlighted recently by cases of human morbidity and mortality linked to acetyl fentanyl abuse. Unfortunately, the lack of information available on the toxicology and metabolism of acetyl fentanyl precludes its detection in human samples. The following study was conducted to test a new analytical procedure for the simultaneous quantification of acetyl fentanyl and its predicted metabolite, acetyl norfentanyl, in human urine. Metabolic reference standards and deuterium-labeled internal standards were synthesized for use in an assay that coupled solid-phase extraction (SPE) with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The accuracy (% Relative Error <5%) and inter- and intrarun precision (%CV <20%) of this new method resulted in low levels of quantification (∼1 ng/mL). Similar results were obtained using liquid chromatography columns manufactured with phenyl-hexyl and biphenyl stationary phases (r(2) > 0.98). Preliminary human liver microsomal and in vivo rodent studies demonstrated that acetyl fentanyl is metabolized by cytochrome P450s to acetyl norfentanyl. Urine samples from rats treated with a toxic dose of acetyl...Continue Reading

References

Oct 1, 1981·Analytical Chemistry·T C KramA C Allen
Sep 6, 2005·The American Journal of Emergency Medicine·William A WatsonKathleen M Wruk
Dec 17, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Ludwig PergerKarin Fattinger
Jul 2, 2009·Mayo Clinic Proceedings·Howard S Smith
Nov 19, 2010·The New England Journal of Medicine·Susan Okie

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Citations

Feb 7, 2016·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Anders HelanderOlof Beck
Dec 1, 2015·Analytical Chemistry·Sheng TangHian Kee Lee
Sep 22, 2015·Journal of Forensic Sciences·Susan M CunninghamJames C Kraner
Apr 29, 2015·Journal of Analytical Toxicology·Iain M McIntyreJonathan R Lucas
May 3, 2015·Journal of Medical Toxicology : Official Journal of the American College of Medical Toxicology·Matthew J LozierLauren Lewis
Mar 30, 2017·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Anders HelanderOlof Beck
Jan 29, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Gabriella RodaMichele Dei Cas
Jul 16, 2019·Forensic Sciences Research·Inês TabarraEugenia Gallardo
Dec 30, 2015·Sudebno-meditsinskaia ekspertiza·A B Melent'ev, S S Kataev
Nov 13, 2018·Frontiers in Pharmacology·Marta ConcheiroGail Cooper
May 30, 2019·Frontiers in Chemistry·Joshua KlingbergShanlin Fu
Nov 10, 2017·Therapeutic Drug Monitoring·Simona PichiniFrancesco Paolo Busardò
Feb 1, 2018·American Journal of Clinical Pathology·Li LiuKenichi Tamama
Oct 27, 2018·Frontiers in Pharmacology·Clara Pérez-MañáMagi Farré
Apr 27, 2019·Frontiers in Pharmacology·Maurice WildeRenata Solimini

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