PMID: 15376526Sep 21, 2004Paper

Quantitative measurement of fathead minnow vitellogenin by liquid chromatography combined with tandem mass spectrometry using a signature peptide of vitellogenin

Environmental Toxicology and Chemistry
Fagen ZhangKent B Woodburn

Abstract

Vitellogenin (VTG) has been proposed as a sensitive biomarker of exposure to environmental estrogenic contaminants that induce VTG production in oviparous species. Enzyme-linked immunosorbent assay (ELISA) methods are currently widely used to measure the VTG levels. In this paper, a new liquid chromatography combined with tandem mass spectrometry (LC/ESI-MS/MS) method for the quantitative analysis of VTG in the plasma of fathead minnows exposed to 17alpha-ethinylestradiol (EE2) has been developed. This method includes, first, the selection of the signature peptide, which involves sodium dodecyl sulfate-polyarylamide gel electrophoresis separation, in-gel digestion, LC/ESI-MS/MS analysis with an ion trap mass spectrometer, and sequence determination with the TurboSEQUEST MS/MS database application; second, optimization of the selected signature peptide in multireaction monitor (MRM) mode with a triple quadrupole mass spectrometer; and third, trypsin digestion of plasma and VTG quantitation via MRM-mode LC/ESI-MS/MS. A series of plasma samples from fathead minnows following exposure to EE2 was assayed. A good correlation was found when EE2-induced plasma samples from fathead minnows were analyzed with ELISA and the described new ...Continue Reading

Citations

Apr 27, 2012·Fish Physiology and Biochemistry·V S RawatN Sehgal
Jan 3, 2014·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Katherine Kemler CoadyTroy Alan Marino
Nov 13, 2015·Ecotoxicology and Environmental Safety·Leah G Luna, Katherine Coady
Apr 19, 2006·Ecotoxicology and Environmental Safety·Kathleen M Jensen, Gerald T Ankley
Sep 5, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Chitra SridarPaul F Hollenberg
Oct 30, 2016·Integrated Environmental Assessment and Management·Katherine K CoadyGerald T Ankley
Apr 3, 2008·Rapid Communications in Mass Spectrometry : RCM·Petia ShipkovaMark Sanders
Nov 9, 2004·Journal of Mass Spectrometry : JMS
Mar 6, 2009·Rapid Communications in Mass Spectrometry : RCM·Alejandro M CohenJoseph H Banoub
Jan 12, 2008·Journal of Proteome Research·Vathany KulasingamEleftherios P Diamandis

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