Quantitative measurement of mRNA at different loci within an individual living cell

Ultramicroscopy
Hironori UeharaAtsushi Ikai

Abstract

Asymmetric localizations of cellular proteins and mRNAs are important for cell functions such as division, differentiation and development. The localization of specific mRNA generates cell polarity by controlling the translation sites of specific proteins and thereby restricting their locations to appropriate cellular regions. We have previously reported a novel method based on atomic force microscopy (AFM) for examining gene expression in a single living cell without killing or destroying it. An AFM tip was inserted into a living cell to extract mRNAs, which were analyzed after multiplication by RT-PCR and quantitative PCR. By applying this method, in this study we performed quantitative measurement of mRNA at different loci within individual living cells.

Citations

Mar 15, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Atsushi Ikai
Jun 16, 2009·Nature Methods·Kiyomi TaniguchiHideki Kambara
Dec 11, 2007·Micron : the International Research and Review Journal for Microscopy·Susan G W Kaminskyj, Tanya E S Dahms
Oct 10, 2007·Colloids and Surfaces. B, Biointerfaces·Hyonchol KimAtsushi Ikai
Feb 24, 2007·Biochemical and Biophysical Research Communications·Charles M CuerrierMichel Grandbois
Nov 6, 2009·IEEE Transactions on Nanobioscience·Kousuke NogawaToshio Fukuda
Dec 18, 2007·Pflügers Archiv : European journal of physiology·Charles-Antoine LamontagneMichel Grandbois
Aug 9, 2008·BioTechniques·Peter Scherp, Karl H Hasenstein
Mar 17, 2021·Wellcome Open Research·Alexander G BuryGavin Hudson
Mar 14, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Tomoko OkadaHiroshi Muramatsu

❮ Previous
Next ❯

Related Concepts

Related Feeds

AFM in situ DNA

AFM in situ DNA describes in situ analysis (or study) of DNA using atomic force microscopy. Discover the latest research on AFM in situ DNA here.

Related Papers

Biochemical and Biophysical Research Communications
Sung-Woong HanJun Miyake
Biochemical and Biophysical Research Communications
Charles M CuerrierMichel Grandbois
Proceedings of the National Academy of Sciences of the United States of America
Xing ChenCarolyn R Bertozzi
© 2022 Meta ULC. All rights reserved