Quantitative measurement of phosphopeptides and proteins via stable isotope labeling in Arabidopsis and functional phosphoproteomic strategies

Methods in Molecular Biology
Ning Li

Abstract

Protein phosphorylation is one type of posttranslational modification, which regulates a large number of cellular processes in plant cells. As an emerging powerful biotechnology that integrates all aspects of advantages from mass spectrometry, bioinformatics, and genomics, phosphoproteomics offers us an unprecedented high-throughput methodology with high sensitivity and dashing speed in identifying a large complement of phosphoproteins from plant cells within a relatively short period of time. Needless to say, phosphoproteomics has become an integral portion of life sciences, which penetrates various research disciplines of biology, agriculture, and forestry and irreversibly changes the way by which plant scientists study biological problems.Because phosphorylation/dephosphorylation of protein is dynamic in cells and the amount of phosphoproteins is low, the preservation of a phosphor group onto phosphosite throughout protein purification as well as enrichment of these phosphoproteins during purification has become a serious technical issue. To overcome difficulties commonly associated with phosphoprotein isolation, phosphopeptides' enrichment, and mass spectrometry analysis, we have developed a urea-based phosphoprotein purifi...Continue Reading

Citations

Aug 4, 2016·Journal of Agricultural and Food Chemistry·Chang-Bo TangGuang-Hong Zhou
Mar 25, 2015·Molecular BioSystems·Fiorella A SolariRené P Zahedi

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