Quantitative measurement of serum microRNA-21 expression in relation to breast cancer metastasis in Chinese females

Annals of Laboratory Medicine
Guinian WangQinglu Wang

Abstract

Breast cancer is the most common type of cancer in females. Aberrant expression of microRNA-21 (miR-21) has previously been reported in breast cancer tissue. The aim of this study was to investigate expression levels of serum miR-21 in breast cancer patients and evaluate its prognostic value in Chinese females. Real-time quantitative (RQ)-PCR was used to analyze miR-21 expression in archived serum, tumor tissue, and adjacent normal tissue from 549 participants (326 with breast cancer, 223 without breast cancer). We also analyzed associations between serum miR-21 expression and breast cancer subtypes and patient prognosis. Recurrence and survival were analyzed by using the multivariate Cox proportional hazards model. Expression of miR-21 was significantly higher in breast cancer tissues compared with normal adjacent breast tissues (P<0.001). The 2(-ΔΔCt) values for serum miR-21 in breast cancer patients versus healthy controls were 9.12±3.43 and 2.96±0.73, respectively. Multivariate Cox proportional hazards model suggested that serum miR-21 expression was an independent poor prognostic factor for both recurrence (hazard ratio [HR]= 2.942; 95% confidence interval [CI]=1.420-8.325; P=0.008) and disease-free survival (HR=2.732; 95%...Continue Reading

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Citations

Aug 1, 2015·International Journal of Molecular Sciences·Bodo C Melnik
Apr 30, 2016·International Journal of Molecular Sciences·Erika LarreaCharles H Lawrie
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Methods Mentioned

BETA
PCR

Software Mentioned

SPSS

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